防御酶活性、木质素和总酚含量与 辣椒抗黄瓜花叶病毒的关系

（1中国农业大学农学与生物技术学院蔬菜系，北京 100094； 2山东省烟台农业学校，烟台 264002）

A Study on the Relationship between the Resistance to CMV of Hot Pepper and Related Defendant Enzyme Activity, the Content of Lignins, and Total Phenols

The leaves of hot pepper with different resistance were inoculated with cucumber mosaic virus, then the change in the defendant enzyme activities, the content of total lignins, and phenils were investigated. The rapid increase of relative activity of POD emerged at different times (periods), and all lines showed significant discrepancy, the relative activity of POD increased at 5 days after inoculation; the relative activity of PAL decreased at first, followed by an increasing, the rapid increase of PAL emerged at 15 days after inoculation, and all lines showed significant difference; the relative activity of PPO was increased at first, followed by decreasing, the rapid increase of PPO emerged at 10 days after inoculation, and all lines showed significant difference; the relative activity of SOD decreased at first, then increased, and decreased in the end excluding line 301 and line 302 , the relative activity of SOD was very low at 10 days after inoculation; the relative content of total lignins and phenols rose after inoculation, and all lines showed significant differences. The correlation analysis indicated that POD, PAL, PPO, lignins and total phenols were correlated with resistant tolerance at 5 days, 15 days, 10 days, 20 days respectively after inoculation. SOD was not correlated with resistant tolerance at 10 days after inoculation. Their coefficient is 0.1562. Therefore, after the hot pepper were inoculated with CMV, the changes of POD, PAL, PPO, lignins and total phenols reflected the differences of the tolerances, these results can be used for the screening of resistant-tolerant breeding materials at different times.