家蚕核多角体病毒p10基因的克隆和分子系统进化分析

根据GenBank中家蚕核多角体病毒T3株中p10基因的保守序列,克隆得到了8个不同地区的家蚕核多角体病毒的p10基因,并分别与GenBank中的NPVp10基因进行比对.结果发现p10基因都含有1个213 bp的开放阅读框(ORF)共编码70个氨基酸,8株P10蛋白间氨基酸序列相似性为95%~100%,相似性高.与GenBank中Ac-MNPV和BmNPV的P10蛋白相比,8株BmNPV P10蛋白都缺失了3个保守结构域中的C-端结构域,只含有2个保守结构域,即N-端卷曲螺旋和Pro丰富区.

Cloning and Molecular Phylogenetic Analysis of p10 Gene from Bombyx mori Nucleopolyhedrovirus

According to the sequence of p10 gene of Bombyx mori nuclear polyhedrosis virus(BmNPV) isolate T3,eight p10 genes of BmNPV collected from different areas were cloned and aligned respectively with relevant sequences in GenBankTM database.The open reading frame(ORF) of all p10 genes from the 8 strains of BmNPVs include 213 nucleotides,encoding a putative protein of 70 amino acids.Multiple alignment of the P10 proteins indicated that the similarities between the 8 strains are 95%-100%.The p10 genes in all of the 8 strains of BmNPVs also lacked the C-terminal structure domain when compared with AcMNPV p10 gene and BmNPV p10 gene.Therefore,the p10 genes cloned only contains two conserved domains,namely the N-terminal coiled-coil domain,and a proline-rich domain.