A study of genetic diversity of sesame (<Emphasis Type="Italic">Sesamum indicum</Emphasis> L.) in Vietnam and Cambodia estimated by RAPD markers |
| |
Authors: | Toan Duc Pham Tri Minh Bui Gun Werlemark Tuyen Cach Bui Arnulf Merker Anders S Carlsson |
| |
Institution: | (1) Research Institute for Biotechnology and Environmental Technology, Nong Lam University, Linh Trung ward, Thu Duc district, Ho Chi Minh, Vietnam;(2) Department of Plant breeding and Biotechnology, Swedish University of Agricultural Sciences, P.O. Box 101, 23053 Alnarp, Sweden |
| |
Abstract: | Sesame (Sesamum indicum L.) is a traditional oil crop cultivated throughout South East Asia. To estimate the genetic diversity of this crop in parts
at the region, 22 sesame accessions collected in Vietnam and Cambodia were analyzed using 10 RAPD markers. The 10 primers
generated 107 amplification products of which 88 were polymorphic fragments (83%). Genetic diversity of all populations was
Ht = 0.34 when estimated by Nei’s genetic diversity and species diversity was H′sp = 0.513 when estimated by Shannon diversity index. Genetic distance ranged from 0.03 to 0.43, with a mean genetic distance
of 0.23. The unweighted pair group method with arithmetic averages (UPGMA) cluster analysis for the 22 accessions divided
the material in four groups. The dendrogram revealed a clear division among the sesame accessions based on their geographical
region. Interestingly, some geographically distant accessions clustered in the same group, which might indicate the human
factor involved in the spreading of sesame varieties. The high level of polymorphism shown suggests that RAPD techniques can
also be useful for the selection of parents in sesame (Sesamum indicum L.) breeding program and for cultivar differentiation. |
| |
Keywords: | Cluster analysis Genetic distance Germplasm Polymorphism RAPD Sesamum indicum |
本文献已被 SpringerLink 等数据库收录! |
|