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| 提高牛细管冻精精子活力的试验研究 | |
| 引用本文： | 许红喜,刘晓宁,张洪涛,卫喜明,宋伟红,韩志强,韩欢胜,李伟,杨麒.提高牛细管冻精精子活力的试验研究[J].中国奶牛,2011(20):28-30. |
| 作者姓名： | 许红喜 刘晓宁 张洪涛 卫喜明 宋伟红 韩志强 韩欢胜 李伟 杨麒 |
| 作者单位： | 1. 黑龙江农垦科学院畜牧兽医研究所,哈尔滨,150038 2. 黑龙江省家畜繁育指导站,哈尔滨,150060 3. 东北农业大学,哈尔滨,150030 |
| 基金项目： | 现代农业产业技术体系建设专项 |
| 摘 要： | 本文从细管冻精的3个关键技术环节研究了稀释液的种类对精子活力与存活的影响,确定了细管冻精精子在液氮冷冻度过冰晶期对其损伤最小的最佳熏蒸高度（最佳冷冻温度）和最佳熏蒸时间,并且对成品冷冻的细管冻精不同解冻温度及时间进行研究。由柠檬酸钠和果糖组成的稀释液精子存活时间长,Tris稀释液冷冻后精子活力高于其他配方;牛细管冻精最佳熏蒸距离为2cm,时间影响不显著,7~9min均可;用50℃温水15s解冻精子的活力最高。 |
| 关 键 词： | 牛细管冻精 精子活力 解冻温度 |
Experimental Research on Improving Sperm Motility of Bovine Straw Frozen Semen |
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| XU Hong-xi,LIU Xiao-ning,ZHANG Hong-tao,WEI Xi-ming,SONG Wei-hong,HAN Zhi-qiang,HAN Huan-sheng,LI Wei,YANG Qi.Experimental Research on Improving Sperm Motility of Bovine Straw Frozen Semen[J].China Dairy Cattle,2011(20):28-30. | |
| Authors: | XU Hong-xi LIU Xiao-ning ZHANG Hong-tao WEI Xi-ming SONG Wei-hong HAN Zhi-qiang HAN Huan-sheng LI Wei YANG Qi |
| Institution: | XU Hong-xi1,LIU Xiao-ning2,ZHANG Hong-tao1,WEI Xi-ming1,SONG Wei-hong1,HAN Zhi-qiang1,HAN Huan-sheng1,LI Wei1,YANG Qi3(1.Heilongjiang Animal Husbandry and Veterinary Institute,Harbin 150038,2.Heilongjiang Animal Breeding Center,Harbin 150060,3.Northeast Agricultural University,Harbin 150030) |
| Abstract: | To study the effects of different types of diluents on the sperm motility,select the best diluents formula and the best fumigation height and time（best freezing temperature） for the sperm damage of straw frozen semen across ice crystals period,as well as the best thawing time for the product of straw frozen semen,three experiments were carried out in this study.Results showed that the sperm survival time for diluents formula composed of sodium citrate and fructose was longer than other diluents,the sperm motility of Tris diluents was the best.And the best fumigation height was 2cm.Fumigation time had no significant effects,and the adaptive time was in range between 7~9min.Thawing at the temperature 50℃ and for 15s was the best way to increase sperm motility. |
| Keywords: | Bovine Straw Frozen Semen Sperm Motility Thawing Temperatur |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |