| 三种土壤微生物总DNA提取方法的比较 |
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| 引用本文： | 王丽娜,许修宏,宛煜嵩,金芜军.三种土壤微生物总DNA提取方法的比较[J].广西农业生物科学,2009(2):331-334. |
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| 作者姓名： | 王丽娜 许修宏 宛煜嵩 金芜军 |
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| 作者单位： | 东北农业大学生命科学学院;中国农业科学院生物技术研究所; |
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| 基金项目： | 农业部948课题(2007-Z8)资助 |
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| 摘 要： | 本文对3种常用的土壤微生物总DNA提取方法Martin法、高盐改进法及试剂盒法进行了比较，并通过DNA得率、纯度及16S rDNA V3可变区的PCR扩增结合DGGE法（denatumg gradient gel electrophoresis），分别对3种方法进行评价。结果表明，3种方法提取的DNA均能满足土壤微生物多样性分析的要求。其中试剂盒方法操作简单，提取的DNA质量较高，但DNA得率较低且成本昂贵。Martin法和高盐改进法用时较长，DNA得率较高，纯度较低，但对后续PCR扩增和DGGE分析没有明显影响，且成本低廉。
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| 关 键 词： | 土壤微生物 DNA提取 DGGE |
Comparison of three Extraction Methods of Soil Microbial DNA |
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| Wang Li'na , Xu Xiuhong Wan Yusong Jin Wujun Life Sciences Institute,Northeast Agricultural University,Harbin, Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing.Comparison of three Extraction Methods of Soil Microbial DNA[J].Journal of Guangxi Agricultural and Biological Science,2009(2):331-334. |
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| Authors: | Wang Li'na Xu Xiuhong Wan Yusong Jin Wujun Life Sciences Institute Northeast Agricultural University Harbin Biotechnology Research Institute Chinese Academy of Agricultural Sciences Beijing |
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| Institution: | Wang Li'na 1,2 Xu Xiuhong 1 Wan Yusong 2 Jin Wujun 2 1 Life Sciences Institute,Northeast Agricultural University,Harbin,150030,2 Biotechnology Research Institute,Chinese Academy of Agricultural Sciences,Beijing,100081 |
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| Abstract: | Three commonly used methods for soil microbial DNA extraction including Martin method,improved high-salt concentration method and the kit method were assessed in this paper. The evaluation was based on the yield and purity of extracted total DNA,the amplification of 16S rDNA V3 variable region and the subsequent analysis of amplified products by DGGE(denaturing gradient gel electrophoresis). The results showed that each of three methods of DNA extraction could meet the requirements of analysis of soil micro... |
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| Keywords: | Soil microorganisms DNA extraction DGGE |
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