| 黄瓜抗西瓜花叶病毒性状的序列特征性扩增区域(SCAR)标记 |
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| 引用本文： | 张海英,张洁,毛爱军,张峰,许勇,王永健.黄瓜抗西瓜花叶病毒性状的序列特征性扩增区域(SCAR)标记[J].农业生物技术学报,2009,17(2):312-316. |
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| 作者姓名： | 张海英 张洁 毛爱军 张峰 许勇 王永健 |
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| 作者单位： | 国家蔬菜工程技术研究中心,北京,100097 |
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| 基金项目： | 北京市自然科学基金，国家高技术研究发展计划(863计划)，国家自然科学基金，北京市科委资助项目，北京市优秀人才项目 |
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| 摘 要： | 以抗西瓜花叶病毒 (Watermelon mosaic virus, WMV)的黄瓜（Cucumis sativus）品种秋棚和感该病毒的欧洲八号为亲本，构建了120个F6重组自交系群体。根据苗期抗病接种鉴定结果进行遗传分析，发现黄瓜抗WMV的基因是由隐性单基因控制。利用集团分离分析法和相关分子标记技术，获得的特异片段E-ACT/M-CTT-427与WMV的抗性基因连锁，连锁距离为8 cM。双亲差异片段测序结果显示感病亲本目标片段存在6个T碱基的缺失。根据测序结果设计了1对与目的基因遗传距离为8 cM的序列特征性扩增区域(SCAR)引物，该引物可用于黄瓜抗WMV病毒的分子标记辅助育种。
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| 关 键 词： | 黄瓜 西瓜花叶病毒（WMV） 抗性基因 AFLP SCAR |
| 收稿时间： | 2008-3-18 |
| 修稿时间： | 2008-5-11 |
Sequence-characterized Amplified Region (SCAR) Markers Linked to Watermelon mosaic virus Resistant Gene in Cucumber |
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| ZHANG Hai-ying,ZHANG Jie,MAO Ai-jun,ZHANG Feng,XU Yong,WANG Yong-jian.Sequence-characterized Amplified Region (SCAR) Markers Linked to Watermelon mosaic virus Resistant Gene in Cucumber[J].Journal of Agricultural Biotechnology,2009,17(2):312-316. |
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| Authors: | ZHANG Hai-ying ZHANG Jie MAO Ai-jun ZHANG Feng XU Yong WANG Yong-jian |
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| Abstract: | Markers linked to Watermelon mosaic virus ( WMV ) resistant gene in cucumber （Cucumis sativus） were identified using 120 recombinant inbreed lines (RIL6) made between a resistant and susceptible parent (Qiupeng× No.8 European). Genetic analysis based on virus inoculation at the seedling stage showed that the resistant trait in Qiupeng was controlled by a single recessive locus. The AFLP marker E-ACT/M-CTT-427 was linked to the resistant gene with a genetic distance of 8 cM. The polymorphism came from a 6 bp deletion of poly(T) repeat in the susceptible parent. And this AFLP marker was converted into a co-dominant sequence-characterized amplified region(SCAR) marker which could be useful in marker-assisted breeding for WMV resistance. |
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| Keywords: | AFLP SCAR |
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