| 福建永安市辣椒丛枝病植原体的分子检测及鉴定 |
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| 引用本文： | 刘建密,纪翠红,陈细红,林积秀,黄培枝,严叔平,陈文乐,陈彩霞,张宇翔,牟海青.福建永安市辣椒丛枝病植原体的分子检测及鉴定[J].植物保护,2015,41(3):115-118. |
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| 作者姓名： | 刘建密 纪翠红 陈细红 林积秀 黄培枝 严叔平 陈文乐 陈彩霞 张宇翔 牟海青 |
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| 作者单位： | 1. 中国农业大学植物病理学系, 北京100193; 2. 云南省农业科学院生物技术与种质资源研究所, 昆明650223 |
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| 基金项目： | 国家科技重大专项（2013ZX08003-001）; 云南省科技计划项目（2012CH007） |
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| 摘 要： | 永安地区发病辣椒植株表现小叶、黄化、丛枝、簇芽等症状。利用植原体16SrDNA基因的通用引物R16mF2/R16mR2和R16F2n/R16R2,对发病辣椒植株总DNA进行巢式PCR检测,获得约1.2kb的特异性DNA片段。经测序并在GenBank数据库进行比对分析,共获得4条植原体特定的16SrDNA基因序列(CHY-C4-1、CHYY1-1、CHY-Y7-1、CHY-G1-1)。将测得的4条序列与已报道的植原体序列进行同源性比对,并构建系统进化树,结果显示获得的4条植原体序列均聚类到16SrI组,其中CHY-Y1-1、CHY-Y7-1、CHY-G1-1与16SrI-B亚组植原体聚类到同一支,而CHY-C4-1与已报道的16SrI组内的6个亚组均未聚类到一支,因此建议将CHY-C4-1命名为新的亚组。利用iPhyClassifier在线分析软件对获得的4条植原体序列进行虚拟RFLP分析,结果与进化树获得的结果一致。
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| 关 键 词： | 玉米致死性坏死病 玉米 RT-PCR |
| 收稿时间： | 2014/3/13 0:00:00 |
| 修稿时间： | 2014/4/17 0:00:00 |
Molecular identification of the viruses causing maize lethal necrosis disease in Yuxi, Yunnan Province |
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| Liu Jianmi,Ji Cuihong,Chen Xihong,Lin Jixiu,Huang Peizhi,Yan Shuping,Chen Wenle,Chen Caixi,Zhang Yuxiang,Mou Haiqing.Molecular identification of the viruses causing maize lethal necrosis disease in Yuxi, Yunnan Province[J].Plant Protection,2015,41(3):115-118. |
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| Authors: | Liu Jianmi Ji Cuihong Chen Xihong Lin Jixiu Huang Peizhi Yan Shuping Chen Wenle Chen Caixi Zhang Yuxiang Mou Haiqing |
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| Institution: | 1. Department of Plant Pathology, China Agricultural University, Beijing 100193, China; 2. Institute of Biotechnology and Germplasm Resources, Yunnan Academy of Agricultural Sciences, Kunming 650223, China |
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| Abstract: | Maize lethal necrosis disease is caused by synergistic interaction between Maize chlorotic mottle virus (MCMV) and one or several potyviruses. Severe mosaic, leaf necrosis (even plant lethal necrosis) or rough dwarf were found during a survey on virus diseases in maize carried out in Yuxi District, Yunnan Province in January 2014. RT-PCR, molecular cloning and sequence analysis were performed to identify the viruses present in the sampled plants. The results showed that all of the diseased samples tested were co-infected with MCMV and Sugarcane mosaic virus (SCMV) and one of the plants was co-infected with MCMV, SCMV and Southern rice black-streaked dwarf virus (SRBSDV). |
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| Keywords: | corn lethal necrosis disease maize RT-PCR |
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