| 蔺草成熟种子愈伤组织诱导及植株再生研究 |
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| 引用本文： | 俞超,池慧成,张科军,王忠华.蔺草成熟种子愈伤组织诱导及植株再生研究[J].核农学报,2008,22(6):803-805. |
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| 作者姓名： | 俞超 池慧成 张科军 王忠华 |
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| 作者单位： | 浙江万里学院生物技术研究所,浙江宁波315100 |
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| 基金项目： | 浙江省科技厅支撑和引导计划(2007C22009); 浙江万里学院校级重点项目 |
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| 摘 要： | 以"岗山3号"蔺草成熟种子作为外植体,研究了不同激素浓度及组合对愈伤组织诱导及植株再生的影响。结果表明,在2,4-D浓度为2.0mg/L,6-BA浓度为0.5mg/L时,蔺草种子的愈伤组织诱导率最高,为74.7%;在IAA浓度为0.5mg/L,KT浓度为2.0mg/L时,蔺草种子的愈伤组织分化率最高,为83.3%;IAA与KT的比值对再生芽和再生根形态影响较明显。
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| 关 键 词： | 蔺草种子 激素组合 愈伤组织 植物再生 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
CALLUS INDUCTION AND PLANT REGENERATION FROM MATURE SEEDS OF MAT RUSH |
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| YU Chao,CHI Hui-cheng,ZHANG Ke-jun,WANG Zhong-hua.CALLUS INDUCTION AND PLANT REGENERATION FROM MATURE SEEDS OF MAT RUSH[J].Acta Agriculturae Nucleatae Sinica,2008,22(6):803-805. |
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| Authors: | YU Chao CHI Hui-cheng ZHANG Ke-jun WANG Zhong-hua |
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| Abstract: | The mature seeds of Juncus effusus L.cultivar Gangshan No.3 were used as explants to investigate the effects of different concentrations and combinations of hormones on the callus induction and plant regeneration.The results indicated that the callus induction rate of mat rush's seed was the highest on the medium with MS+ 2.0mg/L 2,4-D + 0.5mg/L BA,and the induction rate reaches 74.7%; the callus differentiation rate was the highest on the medium with MS + 2.0mg/L KT + 0.5mg/L IAA,and the differentiation ra... |
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| Keywords: | mat rush tissue culture induction differentiation |
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