| 高效液相色谱法测定5个杏品种的糖和酸 |
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| 引用本文： | 张丽丽,刘威生,刘有春,刘宁,张玉萍.高效液相色谱法测定5个杏品种的糖和酸[J].果树学报,2010(1). |
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| 作者姓名： | 张丽丽 刘威生 刘有春 刘宁 张玉萍 |
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| 作者单位： | 沈阳农业大学园艺学院;辽宁省果树科学研究所; |
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| 基金项目： | 农业部作物种质资源保护项目(NB08-2130135-03);;国家科技基础条件平台重点项目(2005DK21002-19) |
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| 摘 要： | 为了探讨快速准确的测定杏果实中的糖、酸组分及其含量的方法,采用高效液相色谱法测定了5个杏品种果肉中的可溶性糖和有机酸,结果表明杏果肉中的可溶性糖为蔗糖、葡萄糖、果糖、山梨糖醇,其中前3种为主要糖,并且蔗糖含量明显高于其他3种糖的含量;杏果肉中的有机酸包括苹果酸、柠檬酸和奎宁酸,前两种酸为主要酸。利用高效液相色谱法可以成功地测定出杏果实中的糖、酸组分及其含量,并且具有良好的精确度、回收率和重复性。
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| 关 键 词： | 杏 高效液相色谱 糖 有机酸 |
Measurement of sugars,organic acids in 5 apricot cultivars by high performance liquid chromatography |
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| ZHANG Li-li,LIU Wei-sheng,LIU You-chun,LIU Ning,ZHANG Yu-ping.Measurement of sugars,organic acids in 5 apricot cultivars by high performance liquid chromatography[J].Journal of Fruit Science,2010(1). |
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| Authors: | ZHANG Li-li LIU Wei-sheng LIU You-chun LIU Ning ZHANG Yu-ping |
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| Institution: | 1Department of Horticulture;Shenyang Agricultural University;Shenyang;Liaoning 110161 China;2Liaoning Institute of Pomology;Yingkou;Liaoning 115009 China |
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| Abstract: | HPLC (High Performance Liquid Chromatography) method was used for measuring the soluble sugar and organic acid contents of 5 apricot cultivars in this study. Results indicated that the soluble sugars in apricot fruit flesh include sucrose,glucose,fructose and sorbitol,of which the first three were the main sugars,and the content of sucrose was substantially higher than the contents of other three sugars; the organic acids in apricot fruit flesh include malic acid,citric acid and quinine,of which the first t... |
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| Keywords: | Apricot HPLC Sugars Organic acid |
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