Bulked AFLP analysis for the assessment of genetic diversity in white clover (Trifolium repens L.) |
| |
Authors: | R Kölliker ES Jones MZZ Jahufer JW Forster |
| |
Institution: | (1) Plant Biotechnology Centre, Agriculture Victoria, La Trobe University, Bundoora, Victoria, 3083, Australia;(2) Present address: Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zurich, Switzerland;(3) Pastoral and Veterinary Institute, Agriculture Victoria, Hamilton, Victoria, 3300, Australia |
| |
Abstract: | The use of bulked leaf samples from individual plants for amplified fragment length polymorphism (AFLP) analysis was evaluated
as a tool for assessment of genetic diversity in white clover (Trifolium repens L.). Bulking of leaf samples produced slightly simpler AFLP profiles compared to the combined profiles of individual plants
from the same cultivar. Approximately 90% of bands which were present in individual plants were present in bulked samples
of the same cultivar. The majority of those absent were rare bands, shared by less than 25% of individual plants. Replicate
bulk samples gave almost identical banding patterns, demonstrating the robustness of the bulked AFLP technique. Cluster analysis
of AFLP data derived from individual plants resulted in a phenogram similar to that produced from data derived from bulked
samples of the same plants. AFLP analysis of bulked samples detected significant amounts of genetic variability among 52 cultivars
and accessions with genetic similarity values ranging from 0.42 to 0.92. However, cluster analysis of AFLP data only partially
reflected the geographic origin of cultivars and accessions and was not congruent with cluster analysis based on variation
for morphophysiological characters. Bulked AFLP analysis provides a powerful tool for rapid assessment of genetic variability
in white clover and may also be used for cultivar identification.
This revised version was published online in August 2006 with corrections to the Cover Date. |
| |
Keywords: | AFLP bulked analysis genetic diversity molecular marker Trifolium repens L |
本文献已被 SpringerLink 等数据库收录! |
|