噬菌体展示技术筛选副猪嗜血杆菌外膜蛋白P5的抗原表位

为了研究副猪嗜血杆菌（HPS）外膜蛋白P5的抗原表位，应用噬菌体展示随机12肽库，以抗HPS外膜蛋白P5的单克隆抗体作为固相分子，进行了3轮筛选。对随机挑选出的10个分离间隔良好的噬菌斑进行ELISA和West—ernblot等检验，最终确定了4个各结果均为阳性的优势短肽。将4个短肽序列与GenBank中HPS外膜蛋白p5的序列进行比对分析，发现这些序列没有同源性或同源性较低，但竞争ELISA结果显示这些短肽与单抗的结合都能够被外膜蛋白P5有效的抑制。以上结果显示，通过噬菌体展示技术成功获得了4个HPS外膜蛋白P5的模拟表位，为后期开展以抗原表位为基础的诊断、表位疫苗等研究提供了依据。

Screening of mimotopes for Haemophilus parasuis OMP P5 by phage display library

To screen the mimotopes for Haemophilus parasuis OMP PS, using the monoclonal anti- body molecules against Haemophilus parasuis OMP P5 as solid phase screening to screen phage display 12 random peptide library for three rounds. Ten well-separated plaques were stapped by ELISA and Western blot. Four amino acid sequence were obtained. Comparing with the sequence of Haemophilus parasuis OMP P5 in GenBank, none of them was homologous with OMP PS, but their binding to the monoclonal antibody can be restrained by OMP PS. As a result, four mimo- topes of Haemophilus parasuis OMP P5 were got in this study,which laid a good foundation for further study on the research of diagnosation and new vaccine development.