Production of Induced Secondary Metabolites by a Co-Culture of Sponge-Associated Actinomycetes,Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 |
| |
Authors: | Yousef Dashti Tanja Grkovic Usama Ramadan Abdelmohsen Ute Hentschel Ronald J Quinn |
| |
Institution: | 1.Eskitis Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia; E-Mails: (Y.D.); (T.G.);2.Department of Botany II, Julius-von-Sachs Institute for Biological Sciences, University of Würzburg, Julius-von-Sachs-Platz 3, D-97082 Würzburg, Germany; E-Mails: (U.R.A.); (U.H.) |
| |
Abstract: | Two sponge-derived actinomycetes, Actinokineospora sp. EG49 and Nocardiopsis sp. RV163, were grown in co-culture and the presence of induced metabolites monitored by 1H NMR. Ten known compounds, including angucycline, diketopiperazine and β-carboline derivatives 1–10, were isolated from the EtOAc extracts of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163. Co-cultivation of Actinokineospora sp. EG49 and Nocardiopsis sp. RV163 induced the biosynthesis of three natural products that were not detected in the single culture of either microorganism, namely N-(2-hydroxyphenyl)-acetamide (11), 1,6-dihydroxyphenazine (12) and 5a,6,11a,12-tetrahydro-5a,11a-dimethyl1,4]benzoxazino3,2-b]1,4]benzoxazine (13a). When tested for biological activity against a range of bacteria and parasites, only the phenazine 12 was active against Bacillus sp. P25, Trypanosoma brucei and interestingly, against Actinokineospora sp. EG49. These findings highlight the co-cultivation approach as an effective strategy to access the bioactive secondary metabolites hidden in the genomes of marine actinomycetes. |
| |
Keywords: | co-cultivation induced metabolites sponge-associated actinomycetes NMR fingerprint bioactivity |
|
|