湖北部分肉种鸡群J亚群禽白血病血清学调查及PCR检测

采用间接ELISA和ALV-J特异性PCR方法,对湖北省荆州市的两个肉种鸡场共94只淘汰肉种鸡进行J亚群禽白血病血清学调查和ALV-J前病毒核酸检测.两个鸡场共检测出13份ALV-J抗体阳性血清和11份核酸阳性样品,抗体阳性率分别为13.2%和14.6%,核酸阳性率分别为11.3%和12.2%.血清学调查和PCR检测结果表明,湖北鸡群已存在J亚群禽白血病.

Serological Investigation and PCR Detection for Avian Leukosis Subgroup J in Part of Hubei Meat-type Chicken Flocks

Indirect ELISA and specific PCR of ALV-J were used to do serological investigation and provirus nucleic acid detection for ALV-J of 94 chickens from two meat-type chicken farms in Jingzhou City of Hubei province. 13 positive ant-body sera and 11 positive nucleic acids were detected out from the two farms. The positive rates of antibody were 13.2% and 14.6% respectively, and the positive rates of nucleic acid were 11.3%, 12.2% respectively on the two farms. The results of serological investigations and PCR test showed that ALV-J had already existed in Hubei flocks.