Rapid DNA-based identification of wheat and barley varieties |
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| Affiliation: | 1. Graduate School of Integrated Arts and Sciences, University of Tokushima, Tokushima 770-8502, Japan;2. Faculty of Human Life Science, Shikoku University, Tokushima 771-1192, Japan;1. Biotecgen, c/o Department of Biological and Environmental Sciences and Technologies, University of Salento, 73100 Lecce, Italy;2. CNR – Institute of Sciences of Food Production (ISPA), via Prov. Lecce-Monteroni, 73100 Lecce, Italy;3. Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy;4. Department of the Sciences of Agriculture, Food and Environment, University of Foggia, Foggia, Italy;1. School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;2. Intelligent Control and Compute Vision Lab, Hefei University, Hefei 230601, China;3. Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China;4. School of Medical Engineering, Hefei University of Technology, Hefei 230009, China;1. Institute of Cereal Crop, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China;2. Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100089, P.R.China;1. Huaiyin Normal University, College of Life Sciences, Huaian, Jiangsu, China;2. Huaian Customs District, China;3. Nanjing Customs District, China |
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| Abstract: | Micro-fluidic capillary electrophoresis methodology was developed to analyse grain DNA composition, thus to provide unequivocal distinction between varieties of wheat (Triticum aestivum L.) and of barley (Hordeum vulgare L.). This ‘Lab-on-a-chip’ technology complements protein composition analysis by micro-fluidic capillary electrophoresis, which is already in routine use for variety identification. Whereas it had been difficult to distinguish between some varieties by protein analysis using the Lab-on-a-chip system, distinctions proved to be possible using a combination of DNA extraction and microsatellite analysis, taking advantage of the speed and convenience of DNA chips. Several combinations of microsatellites permitted the DNA analysis system to provide distinction between two wheat varieties and between all but two (Chebec and Schooner) of the main eleven Australian barley varieties (Arapiles, Baudin, Barque, Chebec, Gairdner, Grimmett, Lindwall, Parwan, Schooner, Skiff and Sloop). |
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