| 断裂剂加入顺序对DNA交联检测的影响 |
| |
| 引用本文： | 赵琳娜,陈薛钗,钟艳艳,侯庆霞,钟儒刚.断裂剂加入顺序对DNA交联检测的影响[J].安徽农业科学,2013(7):2848-2849,2856. |
| |
| 作者姓名： | 赵琳娜 陈薛钗 钟艳艳 侯庆霞 钟儒刚 |
| |
| 作者单位： | 北京工业大学生命科学与生物工程学院,北京,100124;北京工业大学生命科学与生物工程学院,北京,100124;北京工业大学生命科学与生物工程学院,北京,100124;北京工业大学生命科学与生物工程学院,北京,100124;北京工业大学生命科学与生物工程学院,北京,100124 |
| |
| 摘 要： | 目的]探讨单细胞凝胶电泳技术在检测DNA交联时断裂剂加入顺序及剂量对检测结果的影响。方法]采用抗肿瘤药物卡莫司汀(BCNU)作为DNA交联剂,对DNA断裂剂过氧化叔丁醇(tBHP)的加入顺序和最佳使用剂量进行分析。将DNA拖尾长度作为检测指标,并采用t检验分析。结果]BCNU染毒前加入tBHP组与BCNU染毒后加入tBHP组相比,统计学上均无显著差异。此外,随着tB-HP浓度的增加,DNA断裂程度随之增加,且在400μmol/L时断裂效果非常显著。结论]断裂剂tBHP的加入顺序不会对DNA交联检测的试验结果产生影响;tBHP在浓度为400μmol/L时断裂效果非常显著。
|
| 关 键 词： | 单细胞凝胶电泳 DNA交联 过氧化叔丁醇 卡莫司汀 |
Effect of Different Breaking Agents Exposed Order on DNA Crosslinking Detection |
| |
| Institution: | ZHAO Lin-na et al(College of Life Science and Bioengineering,Beijing University of Technology,Beijing 100124) |
| |
| Abstract: | Objective] The aim was to determine whether the concentration and addition sequence of DNA breaking agent have effect on DNA crosslinking detection in Single Cell Gel Ectrophoresis(SCGE).Method] Carmustine(BCNU),a tumor drug,was used as DNA crosslinking agent,and tert-butyl hydroperoxide(tBHP) as DNA breaking agent.The effect of addition sequence and concentration of tBHP on BCNU DNA crosslinking was assessed by the Tail-length in SCGE.T-test was performed to indicate statistical significance.Result] There was no significant difference between the tBHP pre-treatment and post-treatment in tail-length.Moreover,the DNA fracture was increased with the rise of tBHP concentration,and 400 μmol/L showed a marked DNA breaking effect.Conclusion] tBHP addition sequence has no effect on DNA crosslinking detection,and concentration 400 μmol/L was the best dose in DNA breaking. |
| |
| Keywords: | Single cell gel electrophoresis DNA cross-linking tBHP BCNU |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
