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| 一株耐高温抑菌黏质沙雷氏菌的鉴定及其红色素的初步分离 | |
| 引用本文： | 赵银娟,薛斌,李桂娥,吴小扁. 一株耐高温抑菌黏质沙雷氏菌的鉴定及其红色素的初步分离[J]. 安徽农业科学, 2013, 0(7): 2829-2831 |
| 作者姓名： | 赵银娟 薛斌 李桂娥 吴小扁 |
| 作者单位： | 南京林业大学森林资源与环境学院,江苏省有害生物入侵与控制重点实验室,江苏南京210037;南京大学医学院,江苏南京,210037 |
| 基金项目： | 国家自然科学青年基金，博士点基金，江苏高校优势学科建设工程资助项目 |
| 摘 要： | [目的]为了了解黏质沙雷氏菌的抑菌机理及温度对其产生色素的影响。[方法]从土壤中分离纯化得到1株产红色素细菌,对该菌进行生理生化和16S rDNA鉴定,同时对该菌产生的红色素进行了初步的探讨。[结果]该菌为黏质沙雷氏菌(Serratia marcescens),在28℃条件下培养红色素产量最高,37℃下仍能产生色素,说明该菌株是耐高温红色素产生菌。紫外全波长扫描分析和薄板层析结果表明,其红色色素有可能是灵菌红素。[结论]该菌对霉状杆菌和镰刀菌等病原菌具有一定的抑制作用。 |
| 关 键 词： | 红色素 黏质沙雷氏菌 鉴定 |
Identification of Thermo-stability Serratia marcescens Strain Inhibiting Bacterial and Preliminary Isolation of Red Pigment |
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| Affiliation: | ZHAO Yin-juan et al(Jiangsu Key Laboatory for Prevention and Management of Serratia marcescens on Invation Species,College of Forestry Resources and Environment,Nanjing Forestry University,Nanjing,Jiangsu 210037) |
| Abstract: | [Objective]The research aimed to study the antibacterial mechanism of Serratia marcescens and the effects of the temperature on the production of the red pigment.[Method] A bacterial strain producing red pigment was isolated from soil,and its morphology,biochemical,physiological characteristics and 16S rDNA sequence were investigated.[Result] The strain was identified as Serratia marcescens.The red pigment was extracted by ethyl alcohol.The maximum production was obtained at 28 ℃,still,a little pigment were synthesized at 37 ℃.This result indicated that the strain was temperature-stability.The results of ultraviolet full wavelength scanning analysis and sheet chromatography showed that the red pigment might be prodigiosin.[Conclusion] Serratia marcescens could inhibit bacterial such as fusarium and bacillus cereus mycoides. |
| Keywords: | Red pigment Serratia marcescens Identification |
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