| 采用CTAB与SDS法提取白术DNA的研究 |
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| 引用本文： | 黄恒,王晓丽.采用CTAB与SDS法提取白术DNA的研究[J].安徽农业科学,2013(7):2860-2861. |
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| 作者姓名： | 黄恒 王晓丽 |
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| 作者单位： | 贵阳医学院药学院,药植与生药教研室,贵州贵阳550004;贵阳医学院药学院,药植与生药教研室,贵州贵阳550004 |
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| 摘 要： | 目的]获取白术中高质量基因组DNA。方法]选取新鲜白术叶片,破碎细胞壁,用经改良的CTAB与SDS法进行DNA分离纯化提取。结果]改良CTAB法提取的DNA OD值为1.8～1.9,较改良SDS法的OD值(1.5～1.6)高。结论]改良CTAB法提取的DNA浓度和纯度较高,可作为模板用于后续分子标记的研究。
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| 关 键 词： | 白术 CTAB SDS |
Extracting Atractylodes macroephala DNA by Using CTAB and SDS Method |
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| Institution: | HUANG Heng et al(Department of Pharmaceutical Plant and Pharmacognosy,Medical College of Guiyang Medical University,Guiyang,Guizhou 550004) |
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| Abstract: | Objective] To obtain high-quality DNA from Atractylodes macroephala.Method] Selecting fresh leaf of A.macroephala,broken cell wall,CTAB and SDS method were adopted to conduct DNA isolation and purification.Result] The results showed that OD value by using improved CTAB method is 1.8-1.9,which is higher than OD value 1.5-1.6 by using improved SDS method.Conclusion] DNA concentration and purification by using CTAB method is higher,which can be used as templet in subsequent molecular markers. |
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| Keywords: | Atractylodes macroephala CTAB SDS |
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