Diagnosis of canine brucellosis by ELISA using an antigen obtained from wild Brucella canis |
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| Affiliation: | 1. Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA;2. The Intracellular Pathogens Research Laboratory, Comparative Medicine Institute, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, USA;3. Faithful Friends Animal Clinic, Rogers, AR 72758, USA;1. Department of Veterinary Sciences and Public Health, University of Milan, Via Celoria 10, Milano 20133, Italy;2. Veterinary Animal Hospital Città di Pavia, Pavia, Italy;3. Department of Veterinary Medical Sciences, Alma Mater Studiorum – University of Bologna, Ozzano dell''Emilia (BO), Italy |
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| Abstract: | An indirect ELISA test was developed for the diagnosis of Brucella canis infection in dogs. A bacterial whole cell extract was used as a solid phase antigen, using B. canis isolated from an infected animal. Sera from culture-positive and healthy negative animals were used as internal reference controls. The cut-off point was determined by a mathematical formula for a statistically valid value, which defined the upper prediction limit, based on the upper tail of the t-distribution of 21 negative control sera readings, for the confidence level of 99.5%. The sensitivity and specificity of the ELISA test were 95% and 91%, respectively. The ELISA test showed a significant concordance index (K = 0.84) with the agar gel immunodiffusion test. The reliability of the ELISA for the detection of infected animals was established by a double blind study testing 280 sera provided by serum banks from different diagnostic and research institutions and analyzed by ROC Curve. |
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