Phenotypic and genetic characterization of Salmonella enterica subsp. enterica serovar Typhimurium isolated from pigs in Rio Grande do Sul,Brazil |
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| Institution: | 1. Department of Bacteriology & Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;2. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran;3. Departments of Epidemiology and Biostatistics, School of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran;1. Department of Biology, University of Vermont, Burlington, VT, United States;2. Laboratorio de Entomología Aplicada y Parasitología, Escuela de Biología, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala, Edificio T-10 Ciudad Universitaria Zona 12, Ciudad de Guatemala, Guatemala;3. Statistical Software Support and Consulting Services, University of Vermont, Burlington, VT, United States;1. PhD course in Molecular Medicine, University of Palermo, Palermo, Italy;2. Department of Sciences for Health Promotion and Mother-Child Care “G. D''Alessandro”, University of Palermo, Via del Vespro 133, I-90127 Palermo, Italy;3. Toplab University of Applied Science Leiden, Zernikedreef 11, 2333 CK Leiden, Netherlands |
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| Abstract: | This study aimed to investigate the relatedness of porcine Salmonella enterica subsp. enterica (S.) serovar Typhimurium strains isolated in Southern Brazil. Sixty-six isolates from pigs belonging to three commercial companies were submitted to phage typing, XbaI-macrorestriction (PFGE), IS200 hybridization, rep-PCR, antimicrobial susceptibility testing, and PCR assay targeting the spvR region. All strains presented a unique rep-PCR pattern and 63 strains had a common IS200 profile. One pulse-type (XA) was the most prevalent (39/66 strains) and included strains of phage types DT177, DT192, DT194 and RDNC. The spvR region was detected in three strains, which harboured plasmids of 90 kb. High rates of tetracycline, sulfonamide and streptomycin resistance were found. Isolates from farms located in different geographic regions but associated to the same commercial companies clustered together and presented a common resistance profile. Results suggested that clonal groups of S. Typhimurium are present in pig commercial companies in Southern Brazil. |
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