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| 应用RAPD技术对中国3品系实验用小型猪进行亲缘关系的分析 | |
| 引用本文： | 吴丰春,魏泓,甘世祥,王爱德,曾养志.应用RAPD技术对中国3品系实验用小型猪进行亲缘关系的分析[J].畜牧兽医学报,2001,32(6):487-491. |
| 作者姓名： | 吴丰春 魏泓 甘世祥 王爱德 曾养志 |
| 作者单位： | 1. 第三军医大学实验动物中心, 2. 贵阳中医学院 3. 广西大学 4. 云南农业大学 |
| 基金项目： | 国家自然科学基金重点项目(39830190);国家“九五”重点科技攻关项目(96-23-06-01). |
| 摘 要： | 随机扩增多态ＤＮＡ (ＲａｎｄｏｍＡｍｐｌｉｆｉｅｄｐｏｌｙｍｏｒｐｈｉｃＤＮＡ ,ＲＡＰＤ)技术 ,在动物、植物、微生物种属鉴定 ,亲缘关系的划分及多态性分析中得到广泛应用 ,但应用于小型猪方面的研究报道很少 ,尤其是对我国特有的实验用小型猪的研究 ,目前国内外尚未见报道。贵州小型香猪、广西巴马小型猪及西双版纳近交系小耳猪由于其近交程度高、遗传性稳定以及良好的实验应用效果 ,已引起了国内外相关领域的关注。本实验应用 1 0 0条随机引物对上述 3品系小型猪的基因组ＤＮＡ进行ＲＡＰＤ研究 ,旨在筛选具有品系间多态性的ＲＡ… |
| 关 键 词： | 猪 小型猪 亲缘关系 3品系 RAPD技术 遗传 |
| 文章编号： | 0366-6964(2001)06-0487-05 |
PRELIMINARY ANALYSIS ON PHYLOGENETIC RELATIONSHIPAMONG THREE STRAINS OF CHINESELABORATORY MINIATURE PIG BY RAPD TECHNIQUE |
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| WU Feng chun,WEI Hong,GAN Shi xiang,WANG Ai de,ZENG Yang zhi.PRELIMINARY ANALYSIS ON PHYLOGENETIC RELATIONSHIPAMONG THREE STRAINS OF CHINESELABORATORY MINIATURE PIG BY RAPD TECHNIQUE[J].Acta Veterinaria et Zootechnica Sinica,2001,32(6):487-491. | |
| Authors: | WU Feng chun WEI Hong GAN Shi xiang WANG Ai de ZENG Yang zhi |
| Abstract: | The phylogenetic relationship was studied among three strains of Chinese laboratory miniature pig including Guizhou miniature pig,Guangxi Bama pig,Xishuangbanna inbred pig with RAPD of 100 single primers.39 polymorphic primers were selected out,in which 15 single could be used to distinguish among three strains,8 primers among Guizhou miniature pig and the other two,4 primers among Guangxi Bama miniature pig and the other two,12 primers among Xishuangbanna inbred miniature pig and the other two.By using 15 polymorphic primers,RAPD analysis showed the phylogenetic index was 0 780 between Guizhou and Guangxi Bama miniature,0 663 between Xishuangbanna inbred and Guizhou miniature Pigs.The results suggested that phylogenetic relationship was closer between Guizhou and Gangxi Bama miniature pigs than that between Xishuangbanna and the other two miniature pigs. |
| Keywords: | Miniature pig Phylogenetic relationship RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |