| 五指山猪肺炎克雷伯菌肺炎亚种的分离及鉴定 |
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| 引用本文： | 荣 光,赵军明,侯冠彧,王东劲,周汉林.五指山猪肺炎克雷伯菌肺炎亚种的分离及鉴定[J].西北农业学报,2011,20(10):1-5. |
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| 作者姓名： | 荣 光 赵军明 侯冠彧 王东劲 周汉林 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所;中国热带农业科学院科技信息研究所 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项(PZS056) |
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| 摘 要： | 从发病猪场濒死的五指山小猪肝脏、脾脏、小肠中分离到3株革兰氏阴性杆菌,对分离菌株进行形态学、培养特性、生化特性和分子生物学鉴定,确定分离菌株为肺炎克雷伯菌肺炎亚种。利用细菌16S rRNA通用引物对3株细菌基因组DNA进行扩增,均可得到大小为1 503 bp的特异性片段,该序列与GenBank上已经登录的猪源肺炎克雷伯菌序列同源性达99%。动物毒性试验证明其对小鼠有较强的致病性。
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| 关 键 词： | 肺炎克雷伯菌肺炎亚种 分离 鉴定 16SrRNA |
Isolation and Identification of Klebsiella pneumoniae ssp. pneumoniae from Wuzhishan Swine |
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| RONG Guang,ZHAO Junming,HOU Guanyu,WANG Dongjin and ZHOU Hanlin.Isolation and Identification of Klebsiella pneumoniae ssp. pneumoniae from Wuzhishan Swine[J].Acta Agriculturae Boreali-occidentalis Sinica,2011,20(10):1-5. |
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| Authors: | RONG Guang ZHAO Junming HOU Guanyu WANG Dongjin and ZHOU Hanlin |
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| Institution: | 1(1.Tropical Crops Genetic Resources Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou Hainan 571737,China;2.Scientific and Technical Information Institute,Chinese Academy of Tropical Agricultural Sciences,Danzhou Hainan 571737,China) |
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| Abstract: | Three strains of Gram-negative bacillus had been isolated from liver,spleen and small intestine of infected moribund Wuzhishan piglets.These strains were identified by morphological,cultural,biochemical characteristics and technology of molecular biology.They were identified as Klebsiella pneumoniae ssp.pneumoniae by biochemical experimentation.According to 16S rRNA.The 16S rRNA was amplified by PCR with universal primers.A 1 503 bp special segment was obtained from K.pneumoniae isolated from Wuzhishan piglets,Which shared 99% sequence homology with corresponding fragments of K.pneumoniae in GenBank.Animal test indicated the bacterium had high pathogenicity to mice. |
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| Keywords: | Klebsiella pneumoniae ssp pneumoniae Isolation Identification 16S rRNA |
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