小麦禾谷胞囊线虫不同抗性品种中几丁质酶基因Cht4的表达分析

以小麦稳定表达的肌动蛋白(Actin)为对照,利用半定量RT-PCR技术对小麦禾谷胞囊线虫(Heterodera ave nae)接种后在不同抗性品种不同时期不同部位的几丁质酶基因Cht4的表达量进行了分析.结果表明,接种12 h后,Cht4的表达量无明显的增加,抗、感品种之间差异不明显;接种36 h后,抗、感品种中的Cht4的表达量都明显增加,抗性品种中的表达量明显高于感病品种,叶部表达量高于根部的表达量;接种60 h后,抗、感品种中Cht4的表达量都维持在较高的表达水平,抗病品种比感病品种中Cht4的表达量高;接种84 h后,感病品种与接种前相比变化不明显,而抗病品种的表达量仍高于接种前的表达量.

Expression analysis of wheat chitinase gene Cht4 in the resistance and susceptible varieties to cereal cyst nematode

Expression analysis about wheat chitinase gene,in the different growing stages and different positions of different resistance cultivars after CCN inoculation, was carried by semi-Q RT-PCR, using wheat Actin gene as a control. The result showed that the quantity of chitinase gene expression obvious increased after 12 h inoculation, and there were no obvious differences between resistant and susceptible cultivars. After 36 h inoculation, the expression quantity in both cultivars increased obviously, and the quantity in resistant cultivars was more than susceptible cultivars, and in leaves was more than in roots. After 60 h inoculation, the gene expression quantity in resistant and susceptible cultivars was keeping a high level, and the former was more than the latter. The expression in susceptible cultivars after 84 h inoculation didn' t have obvious difference from the ones without inoculation, but the expression quantity in resistant cultivars was still higher than those without inoculation.RT-PCR;chitinase gene