板栗成熟胚再生体系的建立与优化

研究了外植体消毒方法、植物生长调节剂组合对胚萌发和生长的影响。以迁西板栗主栽品种‘燕山早丰’成熟胚为试验材料，采用L₉(3⁴)试验设计对增殖培养基进行筛选。结果表明，二次消毒处理可以有效抑制污染，污染率最低为16.67%。胚萌发和生长最佳基本培养基为WPM+6-BA 1.0 mg·L^-1，萌发率最高达到95.00%，胚萌发苗最高为59.65 mm。不定芽诱导的最适培养基为:MS+6-BA 1.0 mg·L^-1+IBA 0.1 mg·L^-1。芽诱导率最高为92.50%。最适增殖培养基为GD+ZT 2.0 mg·L^-1+NAA 0.1 mg·L^-1，增殖系数最大为3.91。

Establishment and Optimization of in vitro Regeneration System of Mature Embryo of Castanea mollissima

Effects of sterilization method and the combination of plant growth regulators on the embryo germination and growth of Castanea mollissima were examined by taking the cultivar ‘Yanshanzaofeng’ from Qianxi County as test material, and using L₉(3⁴) orthogonal experimental design. It was found that the two-time sterilization treatment could effectively inhibit the contamination, and the lowest rate of contamination was 16.67%. The optimum medium for embryo germination and its growth was WPM+6-BA 1.0 mg·L^-1, by which the highest germination rate reached 95.00%, seedlings from the embryo were as high as 59.65 mm. The optimum medium for adventitious buds was MS+6-BA 1.0 mg·L^-1+IBA 0.1 mg·L^-1, by which the highest induction rate of adventitious bud was 92.50%. The optimum rearing medium was GD+ZT 2.0 mg·L^-1+NAA 0.1 mg·L^-1, the maximum proliferation factor was 3.91.