牛CXCR1基因编码区单核苷酸多态性的研究

本研究采用巢氏PCR、DNA测序和CRS-PCR方法，研究中国荷斯坦牛、鲁西黄牛、渤海黑牛的CXCR1基因编码区的单核苷酸多态性（SNPs），发现了819（G/A）、995（G/A）和1008（C/T）3个SNPs，其中995（G/A）和1008（C/T）为首次报道的两个位点，995（G/A）导致氨基酸的改变Arg422His。CXCR1基因819（A/G）、995（A/G）、1008（C/T）3个位点在中国荷斯坦牛、鲁西黄牛、渤海黑牛群体的优势等位基因相同，分别为G、G、C，其等位基因频率分别为0.63/0.73/0.71、0.60/0.85/0.71、0.74/0.76/0.71。经χ2适合性检验，鲁西黄牛在995（A/G）和1008（C/T）位点达到Hardy-Weinberg平衡状态（P>0.05）；渤海黑牛的所有位点均达到Hardy-Weinberg平衡状态，荷斯坦牛在3个位点均未达到Hardy-Weinberg平衡状态（P<0.05）。3个品种牛在819（A/G）和1008（C/T）位点均表现为中度多态（0.25

Single Nucleotide Polymorphisms (SNPs) within the Coding Regions of CXCR1 Gene in Bos taurus

The single nucleotide polymorphisms(SNPs) within the coding regions of CXCR1 gene in Holstein cattle and Luxi Yellow cattle and Bohai Black cattle were investigated by nested-PCR, DNA sequencing technology and CRS-PCR method. Sequencing results showed that three SNPs were identified, which they were 819(G/A),995(G/A),1008(C/T). 819(G/A) and 1008(C/T) were nonsense mutations, but 995(G/A) resulted amino acids alteration Arg422His.In Holstein cattle and Luxi Yellow cattle and Bohai Black cattle, G and G and T predominan allele were identical at 819(G/A) and 995(G/A) and 1008(C/T), which were G , G , T and the allelic frequencies were 0.63/0.73/0.71, 0.60/0.85/0.71, 0.74/0.76/0.71 respectivly. Chi-square test indicated that 995(G/A)and 1008(C/T) of Luxi Yellow cattle and all the sites of Bohai Black cattle were in accordance with the Hardy-Weinberg equilibrium (P>0.05), while all polymorphic sites were not meet Hardy-Weinberg equilibrium (P<0.05) in Chinese Holstein cattle. The value of polymorphism information content indicated that 819(G/A) and 1008(C/T)s were moderate polymorphism in three breeds（0.25＜PIC＜0.5）, while 995(G/A) were slight polymorphism in Luxi Yellow cattle and were moderate polymorphism in other two breeds.