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| 猪苓糖肽的HPCE指纹图谱分析 | |
| 引用本文： | 徐燕娟,钱燕娟,蒋小丰,龚胜萍.猪苓糖肽的HPCE指纹图谱分析[J].食用菌学报,2007,14(4):47-50. |
| 作者姓名： | 徐燕娟 钱燕娟 蒋小丰 龚胜萍 |
| 作者单位： | 1. 上海市工业微生物研究所,上海,200233 2. 上海市中医药大学,上海,201203 3. 上海市农业科学院瑞丰农业科技有限公司,上海,201106 |
| 摘 要： | 采用毛细管电泳法(HPCE)建立猪苓糖肽的指纹图谱,HPCE工作条件为:采用电解质溶液50mmol/L硼砂缓冲溶液(pH=10.0),毛细管熔融石英毛细管柱(75μm×75cm,有效长度为66.5cm),电压20kV,温度25℃,波长200nm。猪苓糖肽的HPCE图谱有8个共有峰。该法具有较好的分离效果,可为猪苓糖肽的质量控制提供有效手段。 |
| 关 键 词： | 猪苓糖肽 指纹图谱 |
| 文章编号： | 1005-9873（2007）04-0047-04 |
| 收稿时间： | 2007-03-01 |
| 修稿时间： | 2007-04-15 |
Analysis of Polyporus umbellatus Glucopeptides Using High-performance Capillary Electrophoresis-Fingerprint Spectra (HPCE-FPS) |
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| XU Yanjuan,QIAN Yanjuan,JIANG Xiaofeng,GONG Shengping.Analysis of Polyporus umbellatus Glucopeptides Using High-performance Capillary Electrophoresis-Fingerprint Spectra (HPCE-FPS)[J].Acta Edulis Fungi,2007,14(4):47-50. | |
| Authors: | XU Yanjuan QIAN Yanjuan JIANG Xiaofeng GONG Shengping |
| Abstract: | High-performance capillary electrophoresis(HPCE)using a fused quartz capillary column was employed for fingerprint spectrum analysis of Polyporus umbellatus glucopeptide.Eight peaks were found to be constant in all nine glucopeptide samples tested.The technique represents a practical method for separating and identifying P.umbellatus glucopeptide,and our data provide the basis for effective quality control of glucopeptide material from this fungus. |
| Keywords: | HPCE |
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