| 液相色谱-电喷雾质谱联用检测蜂蜜中四种硝基呋喃类代谢物 |
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| 引用本文： | 蒋原,丁涛,沈崇钰,陈惠兰.液相色谱-电喷雾质谱联用检测蜂蜜中四种硝基呋喃类代谢物[J].畜牧与兽医,2005,37(3):12-15. |
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| 作者姓名： | 蒋原 丁涛 沈崇钰 陈惠兰 |
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| 作者单位： | 1. 南京农业大学动物医学院,江苏,南京,210095;江苏出入境检验检疫局,江苏,南京,210001
2. 江苏出入境检验检疫局,江苏,南京,210001 |
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| 摘 要： | 介绍了高效液相色谱电喷雾多级质谱(LC/MSn) 同时快速、准确测定蜂蜜中呋喃西林、呋喃唑酮、呋喃它酮和呋喃妥英4种硝基呋喃类抗生素代谢物。硝基呋喃类代谢物在酸性条件下经过邻硝基苯甲醛衍生化, 液相萃取后经色谱分离, 利用二级质谱进行定性和定量。加标样品平均回收率达到64% ~79%, 定量下限(LOQ) 为0 1~1μg·kg-1, 检测限(LOD) 达到0 05~0 5μg·kg-1。该方法测定结果满足欧盟(EU)对进口动物源性食品中硝基呋喃类抗生素的残留要求。
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| 关 键 词： | 硝基呋喃代谢物 电喷雾多级质谱 蜂蜜 |
| 文章编号： | 0529-5130(2005)03-0012-04 |
Determination of four metabolites of nitrofuran antibacterial drugs in honey by liquid chromatograph-electrospray ionization-mass spectrometry |
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| JIANG Yuan,DING Tao,SHEN Chong-yu,CHEN Hui-Lan.Determination of four metabolites of nitrofuran antibacterial drugs in honey by liquid chromatograph-electrospray ionization-mass spectrometry[J].Animal Husbandry & Veterinary Medicine,2005,37(3):12-15. |
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| Authors: | JIANG Yuan DING Tao SHEN Chong-yu CHEN Hui-Lan |
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| Abstract: | A liquid chromatography-electrospray ionization multi-stage mass spectrometry (LC-ESI-Msn) method was developed for the simultaneous determination of the metabolites of four nitrofuran antibiotics in honey. Sample clean-up and analyte enrichment were performed by Liquid-Liquid extraction with ethyl acetate following combined hydrolysis and derivatization with 2-nitrobenzaldehyde (2-NBA). Limit of detection (LOD) of 0.05~0.5 μg kg -1 and limit of quantitation (LOQ) of 0.1~1 μg kg -1 were achieved using electrospray ionization (ESI) in positive mode. The recovery rates were in the range of 64%～82%. All of the analytical results conformed to the guidelines of European Union. |
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| Keywords: | metabolites of nitrofuran electrospray multi-stage mass honey |
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