| 陕西省PRRSV与CSFV、PCV2、PRV混合感染的检测 |
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| 引用本文： | 王波,张鹏,杨增岐,张淑霞,党如意,刘芳宁,黄建宁,樊荣,刘海生.陕西省PRRSV与CSFV、PCV2、PRV混合感染的检测[J].西北农业学报,2009,18(5):27-30. |
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| 作者姓名： | 王波 张鹏 杨增岐 张淑霞 党如意 刘芳宁 黄建宁 樊荣 刘海生 |
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| 作者单位： | 1. 西北农林科技大学,动物医学院,陕西杨凌,712100
2. 杨凌绿方生物工程有限公司,,陕西杨凌,712100 |
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| 基金项目： | 陕西省"13115"科技创新工程重大科技专项 |
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| 摘 要： | 运用RT-PCR和PCR技术对高热病期间陕西西安、宝鸡、渭南、榆林、汉中、商洛等地区猪场送检的146 份样品进行猪繁殖与呼吸综合征病毒(PRRSV)、猪瘟病毒(CSFV) 、猪圆环病毒2 型( PCV2) 和猪伪狂犬病毒(PRV)检测.结果显示:被检样品中PRRSV的阳性率达46.6%(68/146);PRRSV与CSFV、PCV2 和PRV 3种病毒均有混合感染现象, PRRSV + CSFV混合感染率为25%(17/68),PRRSV +PCV2为19.1%(13/68),PRRSV + PRV为4.4% (3/68),PRRSV + PCV2+ CSFV为14.7%(10/68), PRRSV + PCV2+ CSFV+PRV为2.9%(2/68).结果表明以PRRSV为主要病原的混合感染在所检测的高热病发病猪群的存在是非常普遍的.
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| 关 键 词： | 猪繁殖与呼吸综合征病毒(PRRSV) 混合感染 检测 |
| 收稿时间： | 2009/1/4 0:00:00 |
| 修稿时间： | 2009/4/15 0:00:00 |
Detections of the Co-infection of PRRSV, CSFV, PCV2 and PRV in Shaanxi Province |
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| WANG Bo,ZHANG Peng,YANG Zengqi,ZHANG Shuxi,DANG Ruyi,LIU Fangning,HUANG Jianning,FAN Rong and LIU Haisheng.Detections of the Co-infection of PRRSV, CSFV, PCV2 and PRV in Shaanxi Province[J].Acta Agriculturae Boreali-occidentalis Sinica,2009,18(5):27-30. |
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| Authors: | WANG Bo ZHANG Peng YANG Zengqi ZHANG Shuxi DANG Ruyi LIU Fangning HUANG Jianning FAN Rong and LIU Haisheng |
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| Institution: | College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;Yangling LÜfang Bio-engineering CO. LTD, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China;College of Veterinary Medicine, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | The epidemiological investigation on "Swine High Fever Disease"(SHFD) was carried out in this study.The tissue and serum samples of diseased pigs were collected from some pig farms of Xi''an,Baoji,Weinan,Yulin,Hanzhong,Shangluo in Shaanxi Province during SHFD out breaks.Por cinerepro ductive and respiratory syndrome virus(PRRSV),porcine circovirus type2 (PCV-2),classic swine fever virus (CSFV) and pseudo rabies virus (PRV),four viruses were detected by PCR orRTPCR,The results showed,the prevalence of PRRSV were 46.6% (68/146); the coinfection rates of CSFV and PRRSV,PCV2 and P RRSV,PRV and PRRSV w ere 25% (17/68),19.1% (13/68) and 4.4% (3/68),respectively; t he co inf ection rates of CSFV,PCV2 and PRRSV was 14.7% (10/68); the coinfection rates of CSFV,PCV2,PRV and PRRSV was 2.9% (2/68).All the results suggested that the coinfection of PRRSV and other pathogens is very prevalent in these detected pigs. |
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| Keywords: | PRRSV Mixed-infection Detection |
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