复序橐吾组培再生体系研究

用复序橐吾(Ligularia jaluensis Kom.)种子获得无菌苗,取其叶片为外植体,以MS培养基为基础,通过添加不同浓度和种类的细胞分裂素及生长素,目的是筛选出叶片组织培养的最适培养基。结果表明:种子较好的灭菌时间为75%酒精30 s+1‰升汞4min;初代培养基为MS+6-BA0.5mg/L+2,4-D1.0mg/L,由此获得的愈伤组织致密、颜色绿,具有很好的诱导效果,其诱导率达到90%;继代增殖的最适培养基为MS+6-BA1.0mg/L+NAA0.1mg/L,其增殖倍数高达3.9,并且苗生长状况良好;生根最适培养基为:1/2MS+NAA 0.5mg/L,生根率达100%。

Establishment of micropropagation system of Ligularia jaluensis Kom.

The experiment was based on MS culture media and Ligularia jaluensis Kom. Used explants were from aseptic leavses of the germination seeds in vitro. An appropriate culture medium was obtained via regulating the concentrations of cytokinin and auxin. The optimal sterilization method for seeds of Ligularia jaluensis Kom. was 75% ethanol 30 s+1‰ HgCl_2 4 min. MS+ 6-BA 0.5 mg/L+2,4-D 1.0 mg/L was the best culture medium for the first generation callus, the induced ratio of green compact callus reached 90% with this culture medium. The Propagation Coefficient of 3.9 times indicated that MS+6-BA1.0 mg/L+NAA0.1mg/L was the optimal culture medium for subculture proliferation. The best rooting medium was 1/2MS+NAA0.5 mg/L, the survival rate of the plantlets was shown to be up to 100%.