| 犬附红细胞体PCR检测方法的建立 |
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| 引用本文： | 周勇志,李庄,石磊,刘佩红,丁铲,周金林.犬附红细胞体PCR检测方法的建立[J].中国兽医寄生虫病,2008,16(4):1-4. |
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| 作者姓名： | 周勇志 李庄 石磊 刘佩红 丁铲 周金林 |
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| 作者单位： | 1. 中国农业科学院上海兽医研究所/农业部动物寄生虫学重点实验室,上海,200232
2. 上海市动物疾病预防控制中心,上海,201103 |
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| 基金项目： | 上海市科技兴农重点攻关项目 |
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| 摘 要： | 目的 步建立犬附红细胞体PCR检测方法。方法 据已发表的附红细胞体基因序列，设计了一对特异性引物，以犬附红细胞体基因组DNA和附红细胞体可疑病犬样品DNA为模板，聚合酶链式反应（PCR）扩增，扩增产物经克隆测序分析。结果 CR扩增产物大小为541bp，序列分析表明与GenBank数据中发表的序列一致，表明这套引物成功扩增出目的基因序列，但正常犬血样品DNA和弓形虫、伊氏锥虫、吉氏巴贝斯虫、犬细小病毒、犬瘟热的DNA样品都不能扩增出目的基因片段。结论 研究建立的PCR方法可以用于犬附红细胞体的检测，为犬附红细胞体病的诊断及分子流行病学的调查提供了新的手段。
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| 关 键 词： | 犬 附红细胞体 PCR |
ESTABLISHMENT OF PCR METHOD FOR DETECTING EPERYTHTOZOONOSIS IN DOGS |
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| Institution: | ZHOU Yong-zhi, LI Zhuang, SHI Lei, LIU Pei-hong, DING Chan , ZHOU Jin-lin (1. Key Laboratory for Animal Parnsitology, Ministry of Agriculture/Shanghai Veterinary Research Institute, CAAS Shanghai 200232, China; 2. Shanghai Animal Disease Prevention and Control Center, Shanghai 201103, China) |
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| Abstract: | Objective To establish PCR-based method for the detection of dogs＇ Eperythrozoonosis. Methods A pair of primers were designed according to the published sequence of dogs＇ Eperythrozoon. PCR reaction was taken by the designed primers with genomic DNA of Eperythrozoon and blood samples from the infected dogs with Eperythrozoon as template. PCR product was cloned and sequenced. Results The size of PCR product was 541bp which was identical to the reported sequence in GenBank. No cross-reactivity was detected in DNA samples from normal dog blood, Toxoplasma gondii, Trypanosoma evansi, Babesia gibsoni, Canine distemper virus （CDV） and Canine parvovirus （CPV）. Conclusion The result indicated this PCR assay was suitable for detection of dogs＇ Eperythrozoonosis. It could be used in epidemiological investigation and diagnosis of dogs＇ Eperythrozoonosis. |
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| Keywords: | Detection Eperythrozoon PCR |
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