拟南芥抗氧化突变体筛选条件的建立

目的]建立筛选拟南芥抗氧化突变体的条件。方法]将灭菌的野生型拟南芥种子播种于MS培养基上,4℃层化2 d,并于23℃培养室中垂直放置培养4 d后,将幼苗转移至含有不同浓度的甲基紫精（MV）胁迫培养基中,倒置培养,比较不同MV浓度下根的弯曲生长情况,以根停止弯曲生长的MV浓度作为抗氧化胁迫突变体的筛选条件。结果]野生型拟南芥幼苗的根在MV浓度为0.7μmol/L时受到明显抑制,停止生长;在MV浓度低于0.7μmol/L时能正常生长或被轻微抑制。结论]确定筛选抗氧化突变体的MV浓度为0.7μmol/L。

Establishment of the Screening Conditions of Arabidopsis thaliana Antioxidant Mutants

Objective] The research aimed to establish the condition for screening the Arabidopsis thaliana antioxidant mutants.Method] The sterilized seeds of wild-type Arabidopsis thaliana were sown on MS medium plates.Two days after stratification at 4 ℃,the plates were vertically placed in growth room of 23 ℃.Four days after seed germination,the four-day-old seedlings were transferred to the MS agar medium supplemented with different concentrations of methyl viologen(MV) and the plates were inverted with the roots pointing upward.It identified that the concentration of MV was the condition of screening the mutants,which can inhibited the root growth.Result]The growth of the roots of Arabidopsis thaliana seedlings were significantly inhibited and stopped when the concentration of MV was 0.7 μmol/L,while the concentration was lower than 0.7 μmol/L,the plants can grown normally or slightly inhibited.Conclusion]0.7 μmol/L of MV was the concentration for screening antioxidant mutants.