2种叶型元胡叶片的显微特征及主成分分析

目的]比较2种叶型的元胡（Corydalis yanhusuo W.T.W ang）（大叶元胡与小叶元胡）叶片在显微结构上的区别,为元胡的分类提供相关依据。方法]采用石蜡切片法和临时装片法分别制作元胡的叶片横切片和表皮细胞临时装片,利用光学显微镜进行观察,并对其显微特征参数进行主成分分析。结果]大叶元胡的叶片和叶肉组织厚度显著高于小叶元胡;其维管组织在数量上和大小上均优于小叶元胡;大叶元胡叶下表皮细胞垂周壁为深波状,气孔器呈椭圆形,小叶元胡下表皮细胞垂周壁为平直状,气孔器呈近圆形;大叶元胡的主成分值显著高于小叶元胡。结论]大、小叶元胡叶片的显微结构存在显著差异。

Leaf Microstructure of Two Leaf Types Corydalis yanhusuo and Principal Component Analysis of Microscopic Parameters

objective]To compare leaf microstructure differences of the two leaf types Corydalis yanhusuo(big leaf type and small leaf type),so as to provide basis for the classification of Corydalis yanhusuo.Method]The leaf transverse anatomic and epidermal characters were observed with the light microscope under the condition of paraffin section and temporary mount,and the microscopic parameters were analyzed with principal component analysis.Result]The leaf thickness and mesophyll thickness of big leaf type were significant thicker than that of small leaf type;the quantity and size of vascular bundles of big leaf type ones were more than that of small leaf type;the anticlinal wall of leaf lower surface was sinulate in big leaf type,but it was straight in small leaf type;the stomatal apparatuses of big leaf type were oval,while rounded in small leaf type;the principal component values of big leaf type were higher than that of small leaf type.Conclusion]There were significant differences between big leaf type and small leaf type Corydalis yanhusuo in leaf microstructure.