The use of visual marker genes as cell-specific reporters of Agrobacterium-mediated T-DNA delivery to wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.). |
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Authors: | Alex C McCormac Huixia Wu Manzhu Bao Yibing Wang Ruiji Xu Malcolm C Elliott Dong-Fang Chen |
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Institution: | (1) The Norman Borlaug Institute for Plant Science Research, De Montfort University, Scraptoft, Leicester, LE7 9SU, U.K;(2) Department of Forestry, Huazhong Agricultural University, Wuhan, Hubei, 430070, China |
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Abstract: | Transfer of T-DNA from Agrobacterium tumefaciens and A. rhizogenes to cells of wheat (Triticum aestivum L.) and barley (Hordeum vulgare L.) is demonstrated following the inoculation of immature embryos and immature embryo-derived callus. Agrobacterium T-DNA vectors containing the C1/Lc anthocyanin-biosynthesis regulatory genes, the gusA gene or a synthetic green fluorescent protein gene (sgfp-S65T) were constructed from original binary vectors. The visual T-DNA markers were used as cell-autonomous reporters of early Agrobacterium-mediated transformation events in the wheat and barley cells. This localization of the transformed cells revealed a non-random distribution throughout each embryo and callus piece. |
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Keywords: | Agrobacterium barley C1/Lc GFP GUS wheat |
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