基于热胁迫甜玉米雌穗发育差异表达基因的SSR标记开发

为了开发适用于甜玉米抗性遗传的SSR标记，通过对高通量测序基因表达谱技术获取的热胁迫下粤甜13号雌穗发育差异表达基因进行分析，应用生物信息学方法从玉米DNA数据库中开发SSR标记。结果表明，以玉米参考基因数据库获取的58个基因相应序列中，发现53个基因序列存在252个SSR位点，SSR检出率为4.8%，从中开发了251对SSR引物。合成了其中100对SSR引物经PCR及电泳检测，12对引物能在3个甜玉米材料中检测出较好的多态性，占被检测引物的12.0%，扩增带型清晰、稳定性好，这些SSR引物可应用于今后甜玉米抗逆研究中。

Development of SSR Markers in Sweet Corn Using Differentially Expressed Genes of Ears under Heat Stress

Based on the differentially expressed genes in ear development of Yuetian 13 cultivar under heat stress, 58 genes sequences from the database of maize genome, http://maizesequence.org/, were used to develop SSR markers for sweet corn by bioinformatics method. The results indicated that 252 SSRs in 53 of 58 genes sequences were detected using SSR Hunter software, accounting for 4.8%. 251 SSR primers were successfully designed by premier 5.0 software. In further research, 12 of 100 SSR primers showed clear polymorphisms and good stability among 3 sweet corn materials, accounted for 12.0% of all primers detected and could be applied to stress resistance research in sweet corn.