梵净山野生黄连花粉活力研究

采用TTC(2,3,5-氯化三苯基四氮唑)法测定花粉活力,研究了温度、相对湿度、蔗糖含量、硼酸含量等因子对野生黄连花粉活力的影响,以期为探索梵净山野生黄连的生殖机制提供理论依据。结果表明,当温度为26℃、相对湿度为60%时,梵净山野生黄连花粉活力达最大值(45.9%),比温度20℃、相对湿度70%时的花粉活力(10.5%)增加35.4个百分点。随着温度和相对湿度的增加或降低,梵净山野生黄连花粉活力均呈下降的趋势。当蔗糖含量20%、硼酸含量0.006%时,花粉活力为93.4%,当蔗糖含量大于或小于20%,硼酸含量大于或小于0.006%时,梵净山野生黄连的花粉活力均表现为下降。因此,在梵净山野生黄连开花时,控制温度26℃、相对湿度60%,同时喷施20%蔗糖、0.006%硼酸,可以大大提高黄连的花粉活力。

Study on Pollen Vitality of Coptis chinensis Franch.in Fanjingshan Mountain Area

In order to find key factors affecting pollen vitality,and to provide a theoretical basis for exploring the reproductive mechanism of Coptis chinensis Franch. in Fanjingshan mountain area,the influence of temperature,relative humidity,saccharose and element B on pollen vitality of Coptis chinensis Franch. were studied,using TTC(2,3,5-triphenyl tetrazolium chloride) test.The results showed when the temperature was 26 ℃,relative humidity was 60%,pollen vitality reached the maximum(45.9%).Under this condition,pollen vitality was 35.4 percentage points higher than that at 20 ℃ with relative humidity of 70%(10.5%).Pollen vitality of Coptis chinensis Franch. was at a tendency of declining with the temperature and relative humidity increased or decreased.When the concentration of saccharose was 20% and the concentration of H3BO3 was 0.006%,pollen vitality was 93.4%,and it showed a tendency of declining with the concentration of saccharose and H3BO3 increased or decreased.Under temperature of about 26 ℃ and relative humidity of about 60%,with 20% saccharose and 0.006% H3BO3 as well,pollen viability of Coptis chinensis Franch. can be boosted greatly.