反向被动血凝检测鸡传染性支气管炎病毒的研究

本文首次报道一种快速检测鸡传染性支气管炎病毒(IBV)的反向被动血凝法(RPHA),并对其特异性、敏感性和实用性进行了探讨。还用该RPHA法比较了不同纯度抗原制备的抗体的免疫学特性和应用价值。最后,对单克降抗体(McAb)用于RPHA的可能性也进行了一些试验。 结果表明:1.用RPHA法检测IBV有快速、敏感、简便而准确的优点;2.病毒抗原经一次超速离心处理,即可达到RPHA的要求,3.抗体谱较窄或效价较低的McAb,可能不适用于IBV的RPHA方法。

STUDY ON THE DETECTION OF INFECTIOUS BRONCHITIS VIRUS BY REVERSE PASSIVE HEMAGGLUTINATION TEST

A reverse passive hemagglutination(RPHA.test for the detection of infectious bronchitis virus(IBV)is described for the first time in this paper.The specificity,sensitivity and potential for practical application of the RPHA was studied,and the immunological characteristics and usefulness of the antibodies produced by antigens of different purity were also compared.Finally,some work was done to investigate the possibility of using monoclonal antibody(McAb)in the RPHA test.Results showed that the RPHA test was a rapid,sensitive,simple and accurate method for detecting IBV,and that the antigen centrifuged once by ultra-centrifngation could meet the requirements of the RPHA test,whereas the McAb with too narrow an antibody spectrum or with too low a liter might be unsuitable for this test.