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| 苹果离体叶片培养直接体细胞胚胎发生研究 | |
| 引用本文： | 达克东 张松. 苹果离体叶片培养直接体细胞胚胎发生研究[J]. 园艺学报, 1996, 23(3): 241-245 |
| 作者姓名： | 达克东 张松 |
| 作者单位： | 山东农业大学遗传所 园艺系 农学系 |
| 摘 要： | 在ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋ＮＡＡ５０ｍｇ／Ｌ＋２，４＿Ｄ０＼＾５ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的固体培养基上，苹果品种嗄拉试管苗叶片经７天暗培养产生胚性细胞。将具有胚性细胞的叶片转入ＭＳ＋ＢＡ１０ｍｇ／Ｌ＋蔗糖２０ｇ／Ｌ的培养基上暗培养４０天，６５％的叶片直接发生体细胞胚，体细胞胚在同种培养基上萌发，长成小植株。 |
| 关 键 词： | 苹果：离体叶片培养；体细胞胚；小植株 |
Direct Somatic Embryogenesis from in Vitro Leaves of Apple |
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| Da Kedong , Zhang Song, Li Yazhi , and Qi Zengjun. Direct Somatic Embryogenesis from in Vitro Leaves of Apple[J]. Acta Horticulturae Sinica, 1996, 23(3): 241-245 | |
| Authors: | Da Kedong Zhang Song Li Yazhi Qi Zengjun |
| Affiliation: | Da Kedong 1, Zhang Song2, Li Yazhi 1, and Qi Zengjun 3 |
| Abstract: | Embryogenic Cells Were induced from in vitro leaves of apple cultivar `Gala' after 7 days in the dark on MS medium supplemented with 1 0 g/L BA, 5 0mg/L NAA, 0 5mg/L 2,4_D and sucrose 20 0g/L. Sixty five percent of the leaves regenerated somatic embryos directly when transferred to MS medium with 1 0mg/L BA and sucrose 20 0g/L in the dark for 40 days. The regenerated embryos were going to germinate and develop into shoots on the same medium. |
| Keywords: | Apple Leaf culture in Vitro Somatic Embryo shoot |
| 本文献已被 CNKI 维普 等数据库收录！ | |