| 杂色鲍哈维弧茵耐药质粒的鉴定和分析 |
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| 引用本文： | 王瑞旋,耿玉静,冯娟,王江勇.杂色鲍哈维弧茵耐药质粒的鉴定和分析[J].南方水产,2012,8(2):1-6. |
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| 作者姓名： | 王瑞旋 耿玉静 冯娟 王江勇 |
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| 作者单位： | 中国水产科学研究院南海水产研究所,广东省渔业生态环境重点实验室,农业部南海渔业资源开发利用重点实验室,广东广州510300 |
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| 基金项目： | 现代农业产业技术体系建设专项资金，广东省海洋渔业科技推广专项项目，广东省农业攻关项目 |
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| 摘 要： | 文章对引起杂色鲍（Hallotis diversicolor）肌肉萎缩症的病原菌哈维弧菌（Vibrio harveyi）质粒上的磺胺耐药基因进行研究,结果显示,该菌株对复方新诺明完全耐药,其质粒上含有sulⅡ耐药基因。接合转化试验显示该质粒具可转移性,可使受体菌对磺胺制剂复方新诺明产生耐药性,鉴定为耐药质粒,并测定了该耐药质粒的全基因组序列,序列总长为10 940 bp,初步分析显示有7个具有一定功能的ORF框。进一步构建重组表达质粒pR-SET-A-sulⅡ,表达了目的蛋白（31 kDa）。
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| 关 键 词： | 哈维氏弧菌 耐药质粒 sulⅡ基因 |
Identification and analysis of resistant plasmid of pathogenic bacteria Vibrio harveyi isolated from Haliotis diversicolor |
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| WANG Ruixuan
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GENG Yujing
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FENG Juan
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WANG Jiangyong.Identification and analysis of resistant plasmid of pathogenic bacteria Vibrio harveyi isolated from Haliotis diversicolor[J].South China Fisheries Science,2012,8(2):1-6. |
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| Authors: | WANG Ruixuan GENG Yujing FENG Juan WANG Jiangyong |
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| Institution: | (Key Lab. of Fishery Ecology and Environment, Guangdong Province, Key Lab. of South China Sea Fishery Resources Exploitation ＆ Utilzation Ministry of Agriculture, South China Sea Fisheries Research Institute, Ctunese Academy of Fishery Sciences, Guangdong 510300, China) |
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| Abstract: | The pathogenic bacteria Vibrio harveyi discovered in abalone Haliotis diversicolor with withering syndrome is completely re- sistant to trimethoprim-sulfamethoxazole. This study identifies the resistance genes and their location in this bacteria＇s plasmid, suⅡ was detected in the plasmid with polymerase chain reaction. The conjugative transformation test shows that the plasmid is transferable and leads to the resistance of bacteria to sulfonamide. The complete sequence of the R plasmid is 10 940 bp long including 70RF which display biological function, sul Ⅱ was cloned into the multiple cloning sites pRSET-A（ ＋ ） to build a recombinant positive plas- mid. The SDS-PAGE indicates that the recombinant su1Ⅱprotein, in the form of inclusion body, is 31 kDa. |
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| Keywords: | Vibrio harveyi resistant plasmid gene sulⅡ |
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