| 黄瓜EST-SSR位点信息 |
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| 引用本文： | 胡建斌,李建吾.黄瓜EST-SSR位点信息[J].江西农业学报,2008,20(11):58-60. |
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| 作者姓名： | 胡建斌 李建吾 |
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| 作者单位： | 河南农业大学,林学园艺学院,河南,郑州,450002 |
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| 基金项目： | 河南省农业成果转化基金
，
河南农业大学博士启动基金
，
河南农业大学林园学院青年创新基金
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| 摘 要： | 从NCBI网站下载6344条黄瓜EST，通过前处理得到全长为2．91×10^6bp的无冗余EST6033条。利用SSRHunt-er软件从这些序列中搜寻到729个SSR，分布于667条EST中，出现频率为12．08％，分布频率为1／3．99kb。单、二和三核苷酸重复是主导类型，三者共占总数的89．71％。A／T、AG／CT和AAG／CTT分别是单、二和三核苷酸的优势重复基元，分别占总数的29．77％、21．12％和14．95％。黄瓜EST-SSR以4～10次重复为主，基序长度主要集中于12～20bp。最后对这些EST-SSR的多态性潜能进行了评价。
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| 关 键 词： | 黄瓜 EST-SSR 位点信息 |
Information of EST-SSR Loci in Cucumber |
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| HU Jian-bin,LI Jian-wu.Information of EST-SSR Loci in Cucumber[J].Acta Agriculturae Jiangxi,2008,20(11):58-60. |
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| Authors: | HU Jian-bin LI Jian-wu |
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| Institution: | HU Jian - bin, LI Jian - wu (College of Forestry and Horticulture, Henan Agricultural University, Zhengzhou 450002, China) |
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| Abstract: | A total of 6344 ESTs of cucumber were downloaded from the database of NCBI.After pre-processing,6033 non-redundant ESTs with total length of 2.91×106 bp were obtained.By using software SSRHunter,729 SSRs were sought out from these non-redundant ESTs,distributing in 667 ESTs.The frequency of the EST-SSRs was 12.08% and mean distance was 3.99 kb per SSR.Mononucleotide,dinucleotide and trinucleotide repeats were major types,totally accounting for 89.716%.A/T,AG/CT and AAG/CTT were the most frequent motifs,respectively accounting for 29.77%,21.12% and 40.57% in mononucleotide,dinucleotide and trinucleotide repeats.The repeat number of cucumber EST-SSR was mainly 4~10 and motif length of them mostly ranged from 12 bp to 20 bp.Finally,probability of polymorphism of these EST-SSRs was assessed. |
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| Keywords: | EST-SSR |
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