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| 落叶松胚性细胞系分化能力及染色体变异的研究 | |
| 引用本文： | 张清国,梁国鲁,韩素英,胡钠梅,齐力旺.落叶松胚性细胞系分化能力及染色体变异的研究[J].林业科学研究,2010,23(6):877-882. |
| 作者姓名： | 张清国 梁国鲁 韩素英 胡钠梅 齐力旺 |
| 作者单位： | 1. 西南大学园艺园林学院,重庆,400716;中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 2. 西南大学园艺园林学院,重庆,400716 3. 中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京,100091 |
| 基金项目： | 国家863项目(2006AA100109与2008AA10Z126);国家自然科学基金重点项目(30830086)和国家973项目(2009CB119100)以及国家林业局"948"项目(2007-04-03) |
| 摘 要： | 以落叶松未成熟合子胚诱导的16个胚性细胞系为材料,对继代培养的细胞系间及不同世代间染色体数目和体细胞胚胎发生率进行对比研究,结果表明:落叶松胚性细胞系随继代培养时间的增加,总体体细胞胚分化能力逐渐下降,平均体细胞胚分化率从继代培养第7次后的335.6个·g -1降低到第22次后的268.2个·g-1,细胞染色体数目变异率逐步增加,从5.4%上升到40.0%;不同细胞系间体细胞胚分化能力及稳定性差异明显,分化能力较高的细胞系染色体数目变异几率相应较小,在继代培养第25次后,细胞系a214染色体数目变异率为0%,体细胞胚分化率为416.1个·g -1。 |
| 关 键 词： | 落叶松 细胞系 染色体 体细胞胚胎发生 遗传稳定性 |
| 收稿时间： | 2009/3/19 0:00:00 |
Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp. |
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| ZHANG Qing-guo,LIANG Guo-lu,HAN Su-ying,HU Na-mei and QI Li-wang.Somatic Embryogenesis and Multiplicity of Chromosome Number in Embryogenic Cell Lines of Larix ssp.[J].Forest Research,2010,23(6):877-882. | |
| Authors: | ZHANG Qing-guo LIANG Guo-lu HAN Su-ying HU Na-mei and QI Li-wang |
| Institution: | College of Horticulture and Landscape, Southwest University, Chongqing 400716, China; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;College of Horticulture and Landscape, Southwest University, Chongqing 400716, China;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;College of Horticulture and Landscape, Southwest University, Chongqing 400716, China; Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China;Research Institute of Forestry, Chinese Academy of Forestry; Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China |
| Abstract: | The embryogenesis ratios and the chromosome numbers during subculture among sixteen embryogenic cell lines derived from immature zygote of Larix spp. were compared. The results showed a negative correlation between the ability of somatic embryogenesis and the times of subculture, with embryogenesis ratios of 335.6 per gram in the 7th and 268.2 per gram in the 22nd subculture time; and a positive correlation between the embryogenesis ratios and the genetic stability, with chromosome variation ratio of 5.4% in the 7th and 40.0% in the 22nd subculture time. The ability and highly quality of somatic embryogenesis were different among cell lines, however, the most efficient embryogenic cell lines contain the most stability chromosome numbers, with embryogenesis ratio of 416.1 per gram and chromosome variation ratio of 0% in the cell line a214 after subculturing for 25times. |
| Keywords: | Larix cell lines chromosome somatic embryogenesis genetic stability |
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