| 孑遗植物水松基因组DNA提取及ISSR反应体系建立 |
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| 引用本文： | 郑世群,刘金福,吴则焰,洪伟,徐道炜,何中声.孑遗植物水松基因组DNA提取及ISSR反应体系建立[J].福建农林大学学报(自然科学版),2013,42(2). |
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| 作者姓名： | 郑世群 刘金福 吴则焰 洪伟 徐道炜 何中声 |
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| 作者单位： | 1. 福建农林大学林学院
2. 福建农林大学生命科学学院,福建福州,350002 |
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| 基金项目： | 福建省自然科学基金，福建省科技厅资助高校项目 |
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| 摘 要： | 以不同种源水松叶片为材料,应用改良CTAB法提取水松基因组DNA,并对ISSR-PCR反应体系进行优化.结果表明:利用改良CTAB法提取的水松基因组DNA产量高、质量好,符合ISSR分析要求.通过正交试验设计,探讨不同浓度DNA模板、引物、dNPT、Taq DNA聚合酶对水松ISSR-PCR反应体系的影响.
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| 关 键 词： | 水松 DNA提取 ISSR-PCR 体系优化 |
Extraction of genomic DNA and optimization of ISSR reaction system with orthogonal design in Glyptostrobus pensilis |
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| ZHENG Shi-qun
,
LIU Jin-fu
,
WU Ze-yan
,
HONG Wei
,
XU Dao-wei
,
HE Zhong-sheng.Extraction of genomic DNA and optimization of ISSR reaction system with orthogonal design in Glyptostrobus pensilis[J].Journal of Fujian Agricultural and Forestry University,2013,42(2). |
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| Authors: | ZHENG Shi-qun LIU Jin-fu WU Ze-yan HONG Wei XU Dao-wei HE Zhong-sheng |
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| Abstract: | The improved CTAB method was used for the extraction of genomic DNA in different populations of Glyptostrobus pensilis,and a satisfactory ISSR reaction system was also established by orthogonal design.The result showed that the improved CTAB method was suitable for the extraction of DNA,and the DNA extracted by this method had higher yield,better quality,and could be used for ISSR-PCR reaction.A number of factors,which affected the results of ISSR-PCR reaction,such as quantity of template DNA,primer,concentration of dNPT and Taq DNA polymerase were studied. |
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| Keywords: | Glyptostrobus pensilis extraction of DNA ISSR analysis system optimization |
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