土壤微生物多样性实验研究方法概述

对有关土壤微生物多样性,包括微生物类群多样性、群落结构多样性、功能多样性以及基因多样性等的描述与表征方法进行了探讨,同时,对当前国内外土壤微生物多样性的一些实验研究方法,包括土壤微生物分离培养方法、Biolog 微平板方法、FAME 分析方法和分子生物学方法等进行了介绍和评述。并指出在土壤微生物多样性研究中,如果可能的话,需要将各种方法结合起来使用,方可得到有关土壤生物多样性的较为全面的信息和理解。

REVIEW ON LABORATORY METHODS FOR SOIL MICROBIAL DIVERSITY

Methods for describing and characterizing soil microbial diversity, including population diversity, community diversity, gene diversity and function diversity, were discussed in this paper. A series of laboratory methods for measuring soil microbial diversity were reviewed and some new technologies,such as Biolog micro-plates, PFLA analysis and molecular biological techniques (such as PCR, DGGE, RAPD, RFLP, AFLP, SSR) were introduced as well. Meanwhile it was suggested that if possible, it is advisable to combine some of the methods in the test so as to get more accurate and comprehensive information and a better understanding of soil microbial biodiversity.