地衣芽孢杆菌发酵培养基的优化

目的]对地衣芽孢杆菌发酵培养基进行优化，并在此基础上确定最佳培养条件，为实现其工业化生产提供参考。方法]借鉴谷春涛等关于地衣芽孢杆菌TS-1液体培养发酵条件的研究成果，对培养基进行澄清，并运用单因素试验和4因素3水平正交试验对其培养基进行改进，对地衣芽孢杆菌发酵条件进行优化以得到其最佳温度、pH值和接种龄。结果]4种因素对地衣芽孢杆菌发酵影响的显著性次序为：玉米浆〉豆饼粉浸汁〉蛋白胨〉葡萄糖。培养基的最佳配比为：玉米浆0．45g，蛋白胨1．50g，豆饼粉浸汁为1：15，葡萄糖1．50g。最适宜种龄为18h，接种量8％，起始pH值7．5，最佳温度37．5℃，此条件下地衣芽孢杆菌对数生长期在14～20h，发酵周期为22～24h。活菌数每单位提高近1亿个，发酵周期比经验周期缩短10h。结论]试验得出了地衣芽胞杆菌发酵培养基的最佳配比及最佳培养条件，在此条件下培养，收获菌体量大大提高，降低了生产成本，更有利于其大规模工业化生产。

Research on the Optimization of the Medium for Bacillus licheniformis Fermentation

Objective] On the basis of the optimization of the Bacillus licheniformis fermentation medium,the best culture condition was determined for the reference for the implementation of its industrial production.Method] On the basis of the results from Gu Chuntao in the liquid culture fermentation condition of Bacillus licheniformis-TS-1,the further test of the culture medium was conducted,and the experiment in the medium improvement for bacillus licheniformis was done with the single-factor orthogonal test wit...