萝卜RsCYP86MF 基因cDNA全序列克隆及结构特征分析

 本研究通过设计保守的基因特异引物, 运用SMART RACE RT - PCR技术从‘圆白’萝卜中获得一个细胞色素P450基因, 命名为RsCYP86M F, 其cDNA全长1818 bp, 含有1581 bp的完整开放阅读框, 编码526个氨基酸, 该基因编码的蛋白序列与白菜的细胞色素P450基因编码的氨基酸序列同源性高达90% , 且有很高的功能区段保守性; 对其可能编码的蛋白质二级结构进行预测发现, 该蛋白质具有细胞色素P450典型的保守结构域FNAGPRLCIG, 及N - 糖基化位点等结构域。

Cloning and Characterization of Full-length cDNA of RsCYP86MF Gene from Raphanus sativus L.

By SMART RACE RT-PCR strategy with gene specific primers, the full-length cDNA of a cytochrome P450 gene named RsCYP86MF was cloned from Raphanus sativus L.The cDNA was 1818 bp, it encompassed a open reading frame(ORF)with 1581 bp encoding 526 amino acid residues. the sequences of deduced amino acids encoded by cytochrome P450 genes showed the high identity of 90% between Raphanus sativus L.and Brassica campestris subsp. chinensis, and also highly conserved domains of function. It had a conserved domain of cytochrome P450 cysteine heme-iron ligand signature(FNAGPRLCIG), which is typical of cytochrome P450, and the structure domains such as N-glycosylation.