| 纤维素酶辅助法提取柚子皮中多糖的工艺研究 |
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| 引用本文： | 杨喆茗,张益铭,许伟民,王子刚,于海娇,尹春梅.纤维素酶辅助法提取柚子皮中多糖的工艺研究[J].人参研究,2013,25(2):31-34. |
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| 作者姓名： | 杨喆茗 张益铭 许伟民 王子刚 于海娇 尹春梅 |
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| 作者单位： | 1. 吉林农业大学研究生学院 吉林长春·130118
2. 吉林农业大学中药材学院 吉林长春·130118 |
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| 基金项目： | 吉林农业大学中药材学院科研启动基金项目 |
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| 摘 要： | 目的纤维素酶辅助法在柚子皮多糖提取的应用。并筛选出最佳提取条件。方法采取单因素及正交实验的方法，对料液比、反映时间、酶解温度、pH值以及酶含量进行考察，以柚子皮多糖的提取量作为评价指标，找到最佳提取条件。结果酶辅助提取的最佳提取工艺：料液比1：30、反映时间5．5h、酶解温度60℃、pH值5．5、酶含量0．1％。结论纤维素酶辅助法可作为柚子皮多糖提取的一种有效方法。
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| 关 键 词： | 柚子皮 多糖 纤维素酶辅助提取法 提取工艺 |
Study on Extraction Process of Polysaccharide from Grapefruit Peel by Cellulase-assistant Extraction |
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| Authors: | YANG Zhe-ming ZHANG Yi-ming XU Wei-min WANG Zi-gang YU Hai-jiao YIN Chun-mei |
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| Institution: | YANG Zhe-ming HU Tong-tong ZHANG Yi-ming XU Wei-min WANG Zi-gang YU Hai-jiao (Department of Postgraduate, Jilin Agricultural University, Changchun 130118, China) YIN Chun-mei＊ (College of Chinese Medicinal Materials, Jilin Agricultural University, Changchun 130118, China) |
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| Abstract: | Objective To optimize the extraction process of polysaecharide from Grapefruit Peel by Cellulase assistant Extraction. Methods On the basis of single factor experiments, the extraction process was optimized by orthogonal experimental design on effects of the ratio of material to liquid,extracting time,enzyme solution temperature,pH,enzyme levels. The yield of polysac-charide was used to evaluate the factor levels. Results：Enzyme assisted extraction optimum process of extraction technology of polysitccharides： The ratio of material to liquid l：30,extracting time 5.Sh,enzyme solution temperature 60℃ ,pH 5.5,enzyme levels 0.1%. Conclusion Cellulase-assistant Extraction is a kind of effective method to extract polysaccharide from Grapefruit Peel. |
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| Keywords: | Grapefruit Peel Polysaccharide Cellulase-assistant Extraction Extraction process |
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