不同因子对黄秋葵毛状根生长的影响 |
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引用本文: | 宁凝,杨世海.不同因子对黄秋葵毛状根生长的影响[J].人参研究,2013,25(2):20-22. |
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作者姓名: | 宁凝 杨世海 |
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作者单位: | 1. 吉林农业大学中药材学院 吉林长春·130118;中国药材公司 北京·100195 2. 吉林农业大学中药材学院 吉林长春·130118 |
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基金项目: | 吉林省中医药管理局项目 |
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摘 要: | 目的探讨不同因子对黄秋葵毛状根生长的影响。方法利用增殖倍数法测定毛状根的生长.从而明确各因子对黄秋葵毛状根的影响。结果3%蔗糖浓度最适宜毛状根的生长。0.5mg/L6-BA对毛状根有一定的抑制作用,0.5mg/LNAA、IAA对毛状根有一定的促进作用。尤其是0.5mg/LIAA培养的毛状根增殖倍数更为显著。MJA对毛状根生长有抑制作用,SA对毛状根的生长没有明显的影响。结论不同因子不同程度地影响了毛状根的生长,为进一步筛选适宜的黄秋葵毛状根培养体系奠定了基础。
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关 键 词: | 黄秋葵 毛状根 外源激素 诱导子 |
Effects of different factors on the growth of hair roots in Abelmoschus esculentus L.Moench |
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Authors: | Ning Ning Yang Shi-hai |
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Institution: | 1. College of Chinese Medicinal Materials ,Jilin Agricultural University, Changchun 130118,China;2. China National Corp.of Traditional & Herbal Medicine ,Peking 100195, China) |
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Abstract: | Objective: To explore the effects of different factors on the growth of hairy roots in Abelmoschus esculentus L.Moench.Methods: Method of proliferation multiple were used to measure the growth of hairy roots ,and definite the effects of different factors on the growth of hairy roots in A belmoschus esculentus L.Moench.Results: The most suitable sucrose concentration to the growth of hairy roots is 3%. 6-BA of O.5 mg.L-1 has a certain of inhibition to the hairy roots. The NAA and IAA of O.5 mg .L-1 has a certain promotion effect on the hairy roots, especially the multiplication is more notable for which the hairy roots cultivated in the IAA of O.5 mg'L-1. MJA has a certain inhibition to the hairy roots and SA has no effect on the hairy roots.Conclusion: Different factors effect on the growth of hairy roots in different degrees and it lays the foundation for further screening the suitable cultivating sstem of the hairy roots of A belmoschus esculentus L.Moench. |
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Keywords: | Abelmoschus esculentus L Moench Hairy roots Exogenous hormones Elicitors |
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