| 辣椒子叶离体培养再生植株 |
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| 引用本文： | 周钟信 米景九. 辣椒子叶离体培养再生植株[J]. 华北农学报, 1994, 9(2): 59-63 |
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| 作者姓名： | 周钟信 米景九 |
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| 作者单位： | 天津农学院园艺系生物工程研究室，北京农业大学生物学院 |
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| 摘 要： | 随着收集到的８个商品辣椒品种的子叶外植体在ＰＤ培养基上形成芽丛，转移到ＰＥ培养基上伸长为具２－３个节的茎芽，茎芽在ＰＲ培养基上生根，得到了再生植株。芽分化率达１００％，移栽成活率１００％。多植体切除芽后回到ＰＤ培养基上的反复操作可重复诱导芽的分化，提高繁殖系数。
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| 关 键 词： | 辣椒 子叶 再生植株 离体培养 |
In vitro Plant Regeneration from Cotyledon Explants of Capsicum annuum |
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| Zhou Zhongxin, Zhang Zongjiang, Liu Yianjun, Jiang Qianyun. In vitro Plant Regeneration from Cotyledon Explants of Capsicum annuum[J]. Acta Agriculturae Boreali-Sinica, 1994, 9(2): 59-63 |
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| Authors: | Zhou Zhongxin Zhang Zongjiang Liu Yianjun Jiang Qianyun |
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| Abstract: | An efficient program for plant regeneration from cotyledon explants of Capsicum annuum has been developed.Using this program, plant regeneration rate with eight peppercultivars including sweet and chilli type reached 100%.This program can be used in rapid propagation of pepper hybrid as well as genetic transformation via cocultivating of leaf discs with agrobacterium. In the program the organic nutrient components have been improved and the regeneration rate, therefore, has been remarkably raised.The program is:i. shoot buds formation from pepper cotyledon explants on PD medium (20 .days).2. shoot buds elongation to shoot buds with at least two nodes (10 days).3. root formation on PR medium(20 days).4.transplanting plantlets to vormiculite pots and watering with Horland scution. 5.transplanting plants on nutrient soil and culturing in green house. |
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| Keywords: | Capsicum annuum Cotyledon Tissue culture Plant regeneration |
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