乳黄消散对靶动物奶牛的安全性试验

为了确定乳黄消散在奶牛临床上使用的安全剂量,筛选出20头健康奶牛随机分为4组,每组5头,即0倍剂量组(对照组)、1倍推荐剂量组、3倍推荐剂量组和5倍推荐剂量组,每天1次,经口灌服给药连用5d。试验期间,观察受试奶牛的精神状态,采食饮水,眼角、肛门有无污秽物等临床症状,并检测给药后0、3、5d的体温、血常规和生化指标。结果显示,给药后每组奶牛的精神状态良好,采食饮水正常,眼角、肛门无污秽物,且与对照组相比,不同剂量的乳黄消散对受试奶牛的体温、血液生理学指标差异均不显著(P>0.05);给药后第3天,Ⅲ组的ALB、AST、ALT、ALP、LDH、CK和CRE水平显著升高(P<0.05);给药后第5天,ALB、AST、ALT、ALP、LDH、CK、UREA和CRE水平显著升高(P<0.05)。说明乳黄消散至少3倍推荐剂量口服给药对靶动物奶牛是安全的,但5倍推荐剂量对靶动物奶牛的肝、肾有损伤。

Target Animals Safety Test of Ru Huangxiao San in Dairy Cows

The aim of this article is to detect the safe dose of Ru Huangxiao San in healthy dairy cows.Twenty healthy dairy cows were randomly divided into four groups:0-fold dose group(control group),1-fold recommended dose group,3-fold recommended dose group and 5-fold recommended dose group.Each group was given orally once a day for 5 days.During the experiment,the mental state of the cows,drinking water,feculence in the corners of eyes and anus were observed,and the body temperature,blood routine and biochemical indexes were measured at 0,3 and 5 days after administration.The results showed that the cows in each group were in good mental state,intake of drinking water was normal,and there was no contamination in the corners of eyes and anus,and compared with the control group,there were no significant differences in the health status,body temperature and blood physiological indexes of the cows in different doses of Ru Huang Xiao San(P>0.05);on the 3 rd day after treatment,the levels of ALB,AST,ALT,ALP,LDH,CK and CRE of Ⅲ increased significantly(P<0.05);on the 5 th day after treatment,the levels of ALB,AST,ALT,ALP,LDH,CK,UREA and CRE ofⅢincreased significantly(P<0.05).So,Ru Huangxiao San is safe for dairy cows of target animals when given orally at least three times with the recommended dose.However,the recommended dose of 5 times could damage the liver and kidney of dairy cows.