| 花蔺无性系建立的研究 |
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| 引用本文： | 李响,王剑华,李鑫,徐娜,姜长阳. 花蔺无性系建立的研究[J]. 河北农业科学, 2011, 15(3): 82-85 |
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| 作者姓名： | 李响 王剑华 李鑫 徐娜 姜长阳 |
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| 作者单位： | 辽宁师范大学生命科学学院,辽宁大连,116029 |
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| 基金项目： | 辽宁省高等教育教学改革资助项目( 20090304)；辽宁师范大学教学改革资助项目 |
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| 摘 要： | 以花蔺根茎为材料,进行了愈伤组织的诱导和继代、不定芽分化、试管苗生根、移栽定植的研究,建立起根茎无性系。结果表明：根茎愈伤组织诱导培养和继代培养的理想培养基是MS＋BA 0.5 mg/L＋2,4-D2.0 mg/L;愈伤组织颗粒团分化培养的理想培养基是MS＋BA0.5~1.0 mg/L＋NAA0.1 mg/L;试管苗生根培养的理想培养基是1/2 MS＋NAA0.1 mg/L＋IAA0.2 mg/L。试管苗定植容易成活,且长势旺盛,并保持了野生植株的所有生物性状。
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| 关 键 词： | 花蔺 愈伤组织 无性系 快速繁殖 |
Establishment of Regeneration Clone and Rapid Propagation of Botentilla anserrina |
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| LI Xiang,WANG Jian-hua,LI Xin,XU Na,JIANG Chang-yang. Establishment of Regeneration Clone and Rapid Propagation of Botentilla anserrina[J]. Journal of Hebei Agricultural Sciences, 2011, 15(3): 82-85 |
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| Authors: | LI Xiang WANG Jian-hua LI Xin XU Na JIANG Chang-yang |
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| Affiliation: | (College of Life Sciences Liaoning Normal University,Dalian 116029,China) |
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| Abstract: | Using the rhizome of Butomus umbellatus as material,the clone was established including the callus induction and subculture,adventitious bud differentiation,shoot rooting,transplanting and field planting.The results showed that the best medium for callus induction and subculture was MS＋BA 0.5 mg/L＋2,4-D 2.0 mg/L.The best medium for callus grain differentiation was MS＋BA 0.5-1.0 mg/L＋NAA 0.1mg/L.The best media for rooting was 1/2 MS＋NAA 0.1 mg/L ＋ IAA 0.2 mg/L.The stable planted seedlings survived easily,grew well and retained all biological characteristics of the wildness. |
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| Keywords: | Butomus umbellatus Callus Clone Rapid propagation |
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