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Detection and characterization of Clostridium species in soil of Zambia
Authors:Hang'ombe B M  Isogai E  Lungu J  Mubita C  Nambota A  Kirisawa R  Kimura K  Isogai H
Institution:

a Department of Paraclinical Studies, University of Zambia, Lusaka, Zambia

b Department of Preventive Dentistry, Health Sciences University of Hokkaido, Ishikari-Tobetsu 1757, Hokkaido, 061-0293, Japan

c Department of Disease Control, School of Veterinary Medicine, University of Zambia, Box 32379, Lusaka, Zambia

d Department of Virology, Faculty of Veterinary Medicine, Rakuno Gakuen University, Oasa, Ebetsu, 069-0001, Department of Microbiology, Japan

e Sapporo Medical University, Sapporo, 060-0823, Japan

f Division of Animal Experimentation, Sapporo Medical University, Sapporo, 060-0823, Japan

Abstract:In the retrospective study of soil-borne diseases of cattle in Zambia, malignant edema and blackquarter were widespread. One hundred and sixty-five cases with malignant edema and 103 cases with blackquarter were reported between 1985 and 1997. It was found that specific soil-conditions associate the emergence of the soil-borne diseases. Soil samples from five areas in Zambia were examined for the presence of genus Clostridium. Direct immuno-fluorescent assay (IFA) examination showed that C. septicum, C. novyi and C. chauvoei were detected in the soil of specific areas in Zambia, respectively. Causal organisms such as C. perfringens were isolated from the soil samples. The information of area-specific distribution of Clositridium species may give an efficient program in protecting cattle and man.
Keywords:Clostridium  Soil  ZambiaAuthor Keywords: Clostridium  Sol  Zambie
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