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| GNA基因遗传转化甘蔗研究 | |
| 引用本文： | 陈平华,林美娟,薛志平,陈如凯. GNA基因遗传转化甘蔗研究[J]. 亚热带农业研究, 2004, 11(3): 1-6 |
| 作者姓名： | 陈平华 林美娟 薛志平 陈如凯 |
| 作者单位： | 福建农林大学农业部甘蔗生理生态与遗传改良重点开放实验室,福建,福州,350002 |
| 基金项目： | 国家863计划(2001AA241191，2002AA241031)，福建省青年科技人才创新项目(2001J044)，948项目(2003-Q06)资助。 |
| 摘 要： | 虫害常常给甘蔗生产造成重大损失。GNA对蚜虫、飞虱、叶蝉等刺吸式害虫和某些咀嚼式害虫如蛴螬等具有极高的毒性。本研究对引进的GNA基因进行鉴定,并利用基因枪将其转化甘蔗愈伤组织,经筛选、分化,获得了一批抗性再生植株,抽样检测,获得了4株PCR阳性植株,为培育优良抗虫甘蔗新品种创造了条件。 |
| 关 键 词： | GNA基因枪 转化 甘蔗 |
| 文章编号： | 1005-927X(2004)03-0001-06 |
| 修稿时间： | 2004-06-15 |
Studies on GNA gene genetic transformation of sugarcane |
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| CHEN Ping-hua,LIN Mei-juan,XUE Zhi-ping,CHEN Ru-kai Agriculture,P.R.China,Fujian Agriculture and Forestry University,Fuzhou,Fujian ,China). Studies on GNA gene genetic transformation of sugarcane[J]. Subtropical Agriculture Research, 2004, 11(3): 1-6 | |
| Authors: | CHEN Ping-hua LIN Mei-juan XUE Zhi-ping CHEN Ru-kai Agriculture P.R.China Fujian Agriculture Forestry University Fuzhou Fujian China) |
| Affiliation: | CHEN Ping-hua,LIN Mei-juan,XUE Zhi-ping,CHEN Ru-kai Agriculture,P.R.China,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
| Abstract: | Insect pests always cause significant yield losses to sugarcane.It has been proved that snowdrop lectin (Galanthus nivalis agglutinim, GNA) is toxic to aphids,white flies,leafhoppers etc which belong to sap sucking pests and some chewing pests such as grubs. The GNA gene was identified and transformed into calli of sugarcane via particle bombardment in this experiment. Hundreds of Hygromycin-resistant sugarcane seedlings were obtained and four seedlings were PCR-positive in a sample which shows a delighted prospect to select a pest-resistant variety. |
| Keywords: | GNA gene trasformation sugarcane |
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